Histone 3 antibody (H3K18ac)

Catalog No. ABIN2668428

This Rabbit Polyclonal antibody specifically detects Histone 3 in WB, IF, ChIP, ICC, DB and ChIP-seq. It exhibits reactivity toward Human. It has been mentioned in 3+ publications

Quick Overview for Histone 3 antibody (H3K18ac) (ABIN2668428)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K18ac

Purpose: Histone H3K18ac antibody (pAb)

Purification: Protein A Chromatography

Immunogen: This Histone H3 acetyl Lys18 antibody was raised against a peptide including acetyl-lysine 18 of histone H3.

Isotype: IgG

Application Details

Application Notes: ChIP: 10 μg per ChIP ChIP-Seq: 4 μg each ICC/IF: 1 μg/mL dilution WB: 0.5 - 2 μg/mL dilution DB: 0.1 - 0.5 μg/mL dilution

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone 3 antibody (ABIN2668428)

Zhang, Li, Rezaeian, Xu, Chou, Jin, Han, Pan, Wang, Long, Zhang, Huang, Tsai, Tsai, Logothetis, Lin: "H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).

Yasuma, Yasunaga, Takemoto, Sugata, Mitobe, Takenouchi, Nakagawa, Suzuki, Matsuoka: "HTLV-1 bZIP Factor Impairs Anti-viral Immunity by Inducing Co-inhibitory Molecule, T Cell Immunoglobulin and ITIM Domain (TIGIT)." in: PLoS pathogens, Vol. 12, Issue 1, pp. e1005372, (2016) (PubMed).

Cohen, Friedmann, Hwang, Marmorstein, Lee: "The microtubule-associated tau protein has intrinsic acetyltransferase activity." in: Nature structural & molecular biology, Vol. 20, Issue 6, pp. 756-62, (2013) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization.

Molecular Weight: 17 kDa

Gene ID: 3020

NCBI Accession: NP_003522