Histone 3 antibody (H3K4ac)

Catalog No. ABIN2668453

There are 6+ publications for this product available. The Rabbit Polyclonal anti-Histone 3 antibody is suitable to detect Histone 3 in samples from Human. It has been validated for WB, IF, ChIP, ICC, DB and ChIP-seq.

Quick Overview for Histone 3 antibody (H3K4ac) (ABIN2668453)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K4ac

Purpose: Histone H3K4ac antibody (pAb)

Purification: Unpurified

Immunogen: This Histone H3 acetyl Lys4 antibody was raised against a peptide containing acetyl-lysine 4 of histone H3.

Application Details

Application Notes: ChIP: 5 - 10 µL per ChIP ChIP-Seq: 5 - 10 µL each ICC/IF: 1:500 - 1:2,000 dilution WB: 1:1,000 - 1:2,000 dilution

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone 3 antibody (ABIN2668453)

Zhang, Li, Rezaeian, Xu, Chou, Jin, Han, Pan, Wang, Long, Zhang, Huang, Tsai, Tsai, Logothetis, Lin: "H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).

Messier, Gordon, Boyd, Tye, Browne, Stein, Lian, Stein: "Histone H3 lysine 4 acetylation and methylation dynamics define breast cancer subtypes." in: Oncotarget, Vol. 7, Issue 5, pp. 5094-109, (2016) (PubMed).

Valdez, Li, Murray, Ji, Liu, Popat, Champlin, Andersson: "Comparison of the cytotoxicity of cladribine and clofarabine when combined with fludarabine and busulfan in AML cells: Enhancement of cytotoxicity with epigenetic modulators." in: Experimental hematology, Vol. 43, Issue 6, pp. 448-61.e2, (2015) (PubMed).

Hagelkruys, Lagger, Krahmer, Leopoldi, Artaker, Pusch, Zezula, Weissmann, Xie, Schöfer, Schlederer, Brosch, Matthias, Selfridge, Lassmann, Knoblich, Seiser: "A single allele of Hdac2 but not Hdac1 is sufficient for normal mouse brain development in the absence of its paralog." in: Development (Cambridge, England), Vol. 141, Issue 3, pp. 604-16, (2014) (PubMed).

Zhang, Lei, Lu: "Alterations of epigenetic signatures in hepatocyte nuclear factor 4? deficient mouse liver determined by improved ChIP-qPCR and (h)MeDIP-qPCR assays." in: PLoS ONE, Vol. 9, Issue 1, pp. e84925, (2014) (PubMed).

Ma, Pan, Montgomery, Olson, Schultz: "Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 8, pp. E481-9, (2012) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone acetylation is often associated with transcriptional activation. Lysine 4 of histone H3 can also be mono-, di- or trimethylated. This methylation is associated with transcriptional activation.

Molecular Weight: 17 kDa

Gene ID: 3020

NCBI Accession: NP_003522