Histone 3 antibody (H3K36ac)

Catalog No. ABIN2668454

Cited in 3+ publications. The Rabbit Polyclonal anti-Histone 3 antibody (ABIN2668454) specifically detects Histone 3 in WB, IF, ChIP, ICC, DB and ChIP-seq. The antibody is reactive with Human samples.

Quick Overview for Histone 3 antibody (H3K36ac) (ABIN2668454)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K36ac

Purpose: Histone H3K36ac antibody (pAb)

Purification: Unpurified

Immunogen: This Histone H3 acetyl Lys36 antibody was raised against a peptide including acetyl-lysine 36 of histone H3.

Application Details

Application Notes: ChIP: 5 - 10 µL per ChIP ICC/IF: 1:500 - 1:2,000 dilution WB: 1:500 - 1:2,000 dilution The modENCODE and NIH Roadmap Epigenomics Mapping Consortiums have implemented rigorous standardization criteria for all assays and reagents to be used. As part of this initiative, antibody specificity testing and the ability of the antibodies to work in ChIP-Seq were assessed in a large-scale study. This Histone H3 acetyl Lys36 antibody was validated for ChIP-Seq in the study (see reference).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone 3 antibody (ABIN2668454)

Zhang, Li, Rezaeian, Xu, Chou, Jin, Han, Pan, Wang, Long, Zhang, Huang, Tsai, Tsai, Logothetis, Lin: "H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).

Gacias, Gerona-Navarro, Plotnikov, Zhang, Zeng, Kaur, Moy, Rusinova, Rodriguez, Matikainen, Vincek, Joshua, Casaccia, Zhou: "Selective chemical modulation of gene transcription favors oligodendrocyte lineage progression." in: Chemistry & biology, Vol. 21, Issue 7, pp. 841-854, (2014) (PubMed).

Egelhofer, Minoda, Klugman, Lee, Kolasinska-Zwierz, Alekseyenko, Cheung, Day, Gadel, Gorchakov, Gu, Kharchenko, Kuan, Latorre, Linder-Basso, Luu, Ngo, Perry, Rechtsteiner, Riddle, Schwartz, Shanower et al.: "An assessment of histone-modification antibody quality. ..." in: Nature structural & molecular biology, Vol. 18, Issue 1, pp. 91-3, (2011) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone acetylation is often associated with transcriptional activation. Histone H3 Lys36 can also be mono-, di- or trimethylated. Methylation of Histone H3 Lys36 is associated with transcriptional repression.

Molecular Weight: 17 kDa

Gene ID: 3020

NCBI Accession: NP_003522