Histone 3 antibody (H3K122me1)

Catalog No. ABIN2668462

There is 1 publication for this product available. The Rabbit Polyclonal anti-Histone 3 antibody is suitable to detect Histone 3 in samples from Human. It has been validated for WB and DB.

Quick Overview for Histone 3 antibody (H3K122me1) (ABIN2668462)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB)

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K122me1

Purpose: Histone H3K122me1 antibody (pAb)

Purification: Affinity Purified

Immunogen: This Histone H3 monomethyl Lys122 antibody was raised against a peptide containing monomethyl-lysine 122 of human histone H3.

Isotype: IgG

Application Details

Application Notes: WB: 1:250 - 1:1,000 dilution

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone 3 antibody (ABIN2668462)

Ivanauskiene, Delbarre, McGhie, Küntziger, Wong, Collas: "The PML-associated protein DEK regulates the balance of H3.3 loading on chromatin and is important for telomere integrity." in: Genome research, Vol. 24, Issue 10, pp. 1584-94, (2014) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue.

Molecular Weight: 17 kDa

Gene ID: 3020

NCBI Accession: NP_003522