Histone 3 antibody (H3K27me2)

Catalog No. ABIN7540290

There are 6+ publications for this product available. The Rabbit Polyclonal anti-Histone 3 antibody is suitable to detect Histone 3 in samples from Human. It has been validated for WB, IF, ChIP, ICC, DB and ChIP-seq.

Quick Overview for Histone 3 antibody (H3K27me2) (ABIN7540290)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K27me2

Purpose: Histone H3K27me2 antibody (pAb)

Purification: Unpurified

Immunogen: This Histone H3 dimethyl Lys27 antibody was raised against a peptide including dimethyl-lysine 27 of histone H3.

Application Details

Application Notes: ChIP-Seq: 4-6 µL per ChIP ChIP: 5 - 10 µL per ChIP ICC/IF: 1:200 - 1:500 dilution WB: 1:500 - 1:1,000 dilution

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone 3 antibody (ABIN7540290)

Xiao, Shi, Fan, Zhang, Wu, Lan, Minze, Fu, Ghobrial, Liu, Li: "GITR subverts Foxp3(+) Tregs to boost Th9 immunity through regulation of histone acetylation." in: Nature communications, Vol. 6, pp. 8266, (2015) (PubMed).

Herz, Morgan, Gao, Jackson, Rickels, Swanson, Florens, Washburn, Eissenberg, Shilatifard: "Histone H3 lysine-to-methionine mutants as a paradigm to study chromatin signaling." in: Science (New York, N.Y.), Vol. 345, Issue 6200, pp. 1065-70, (2014) (PubMed).

Cie?lik, Hoang, Baranova, Chodaparambil, Kumar, Allison, Xu, Wamsley, Gray, Jones, Mayo, Bekiranov: "Epigenetic coordination of signaling pathways during the epithelial-mesenchymal transition." in: Epigenetics & chromatin, Vol. 6, Issue 1, pp. 28, (2013) (PubMed).

Boulay, Dubuissez, Van Rechem, Forget, Helin, Ayrault, Leprince: "Hypermethylated in cancer 1 (HIC1) recruits polycomb repressive complex 2 (PRC2) to a subset of its target genes through interaction with human polycomb-like (hPCL) proteins." in: The Journal of biological chemistry, Vol. 287, Issue 13, pp. 10509-24, (2012) (PubMed).

Lafos, Kroll, Hohenstatt, Thorpe, Clarenz, Schubert: "Dynamic regulation of H3K27 trimethylation during Arabidopsis differentiation." in: PLoS genetics, Vol. 7, Issue 4, pp. e1002040, (2011) (PubMed).

Qi, Sarkissian, Hu, Wang, Bhattacharjee, Gordon, Gonzales, Lan, Ongusaha, Huarte, Yaghi, Lim, Garcia, Brizuela, Zhao, Roberts, Shi: "Histone H4K20/H3K9 demethylase PHF8 regulates zebrafish brain and craniofacial development." in: Nature, Vol. 466, Issue 7305, pp. 503-7, (2010) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone H3K27 can be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.

Molecular Weight: 17 kDa

Gene ID: 3020

NCBI Accession: NP_003522