Histone H3.X/Y antibody

Catalog No. ABIN2668484

Cited in 1 publication. The Rat Monoclonal anti-Histone H3.X/Y antibody (Clone 8H6-2111) (ABIN2668484) specifically detects Histone H3.X/Y in ChIP, ICC, IF and WB. The antibody is reactive with Human samples.

Quick Overview for Histone H3.X/Y antibody (ABIN2668484)

Target: Histone H3.X/Y

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Application: Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)

Clone: 8H6-2111

Product Details

Purpose: Histone H3.X/Y antibody (mAb) (Clone 8H6-2111)

Purification: Protein G Chromatography

Immunogen: This H3.X/Y antibody was raised against a synthetic peptide corresponding to amino acids 9 - 20 of human H3.X.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified rat IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References

Wiedemann, Mildner, Bönisch, Israel, Maiser, Matheisl, Straub, Merkl, Leonhardt, Kremmer, Schermelleh, Hake: "Identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y." in: The Journal of cell biology, Vol. 190, Issue 5, pp. 777-91, (2010) (PubMed).

Target Details

Target: Histone H3.X/Y

Background: Histones H3.X and H3.Y are primate-specific variants of histone H3. H3.X and H3.Y are roughly 80 % conserved compared to histone H3.3 The H3.Y protein is highly expressed in several primary cell lines, cancer cell lines and human brain. Expression of histone H3.Y is induced by stress. Exogenously expressed H3.X and H3.Y are efficiently incorporated into chromatin and exhibit dynamics similar to canonical histone H3. Knockdown of H3.Y results in slowed growth and altered expression of cell cycle related genes.

Molecular Weight: 17 kDa

Gene ID: 440093