MLL/KMT2A antibody (AA 2829-2883)

Catalog No. ABIN2668494

This Rabbit Polyclonal antibody specifically detects MLL/KMT2A in WB, IF, ICC, IP, ChIP-seq and ChIP. It exhibits reactivity toward Human and Mouse. It has been mentioned in 5+ publications

Quick Overview for MLL/KMT2A antibody (AA 2829-2883) (ABIN2668494)

Target: MLL/KMT2A (MLL) (Myeloid/lymphoid Or Mixed-Lineage Leukemia (MLL))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLL/KMT2A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP)

Product Details anti-MLL/KMT2A Antibody

Binding Specificity: AA 2829-2883

Purpose: MLL / HRX antibody (pAb)

Purification: Protein A Chromatography

Immunogen: This MLL / HRX antibody was raised against a recombinant protein corresponding to amino acids 2829-2883 of human MLL.

Isotype: IgG

Application Details

Application Notes: ChIP: 5 - 10 μg per ChIP ChIP-Seq: 5 - 10 μg each ICC/IF: 0.5 μg/mL dilution WB: 0.5 - 2 μg per ChIP ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-MLL/KMT2A antibody (ABIN2668494)

Kühn, Löscher, Marschalek: "The IRX1/HOXA connection: insights into a novel t(4;11)- specific cancer mechanism." in: Oncotarget, Vol. 7, Issue 23, pp. 35341-52, (2016) (PubMed).

Kang, Piao, Ryu, Kang, Chang, Keum, Hyun: "Interaction of DNA demethylase and histone methyltransferase upregulates Nrf2 in 5-fluorouracil-resistant colon cancer cells." in: Oncotarget, Vol. 7, Issue 26, pp. 40594-40620, (2016) (PubMed).

Ahmad, Katryniok, Scholz, Merkens, Löscher, Marschalek, Steinhilber: "Inhibition of class I HDACs abrogates the dominant effect of MLL-AF4 by activation of wild-type MLL." in: Oncogenesis, Vol. 3, pp. e127, (2014) (PubMed).

Pradeepa, Grimes, Taylor, Sutherland, Bickmore: "Psip1/Ledgf p75 restrains Hox gene expression by recruiting both trithorax and polycomb group proteins." in: Nucleic acids research, Vol. 42, Issue 14, pp. 9021-32, (2014) (PubMed).

Wilkinson, Ballabio, Geng, North, Tapia, Kerry, Biswas, Roeder, Allis, Melnick, de Bruijn, Milne: "RUNX1 is a key target in t(4;11) leukemias that contributes to gene activation through an AF4-MLL complex interaction." in: Cell reports, Vol. 3, Issue 1, pp. 116-27, (2013) (PubMed).

Target Details for MLL/KMT2A

Target: MLL/KMT2A (MLL) (Myeloid/lymphoid Or Mixed-Lineage Leukemia (MLL))

Alternative Name: MLL / HRX

Background: MLL (myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila)), also known as Histone-lysine N-methyltransferase HRX, is a histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1 /MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation. Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9'. Required for transcriptional activation of HOXA9. Promotes PPP1R15A-induced apoptosis.

Molecular Weight: 180 kDa

Gene ID: 4297

NCBI Accession: NP_005924

Pathways: Warburg Effect