DNMT1 antibody (AA 637-651)

Catalog No. ABIN2668504

The Mouse Monoclonal anti-DNMT1 antibody has been validated for WB, IHC, IP and ChIP. It is suitable to detect DNMT1 in samples from Human and Mouse. There are 4+ publications available.

Quick Overview for DNMT1 antibody (AA 637-651) (ABIN2668504)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Clone: 60B1220-1

Product Details anti-DNMT1 Antibody

Binding Specificity: AA 637-651

Purification: Affinity Purified

Immunogen: This DNMT1 antibody was raised against a peptide corresponding to amino acid residues 637-651 of human DNMT1.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-DNMT1 antibody (ABIN2668504)

Liao, Karnik, Gu, Ziller, Clement, Tsankov, Akopian, Gifford, Donaghey, Galonska, Pop, Reyon, Tsai, Mallard, Joung, Rinn, Gnirke, Meissner: "Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells." in: Nature genetics, Vol. 47, Issue 5, pp. 469-78, (2015) (PubMed).

Singh, Archer: "Analysis of the SWI/SNF chromatin-remodeling complex during early heart development and BAF250a repression cardiac gene transcription during P19 cell differentiation." in: Nucleic acids research, Vol. 42, Issue 5, pp. 2958-75, (2014) (PubMed).

Ganguly, Chen, Shin, Devaskar: "Prenatal caloric restriction enhances DNA methylation and MeCP2 recruitment with reduced murine placental glucose transporter isoform 3 expression." in: The Journal of nutritional biochemistry, Vol. 25, Issue 2, pp. 259-66, (2014) (PubMed).

Schnekenburger, Talaska, Puga: "Chromium cross-links histone deacetylase 1-DNA methyltransferase 1 complexes to chromatin, inhibiting histone-remodeling marks critical for transcriptional activation." in: Molecular and cellular biology, Vol. 27, Issue 20, pp. 7089-101, (2007) (PubMed).

Target Details for DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: DNMT1

Molecular Weight: 190 kDa

Gene ID: 1786

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection