POLR2F antibody

Catalog No. ABIN7938437

Cited in 20+ publications. The Mouse Monoclonal anti-POLR2F antibody (Clone 4H8) (ABIN7938437) specifically detects POLR2F in WB, IF, ChIP, ChIP-seq and ICC. The antibody is reactive with Human, Rat, Mouse, C. elegans and Saccharomyces cerevisiae samples.

Quick Overview for POLR2F antibody (ABIN7938437)

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Reactivity: Human, Rat, Mouse, C. elegans, Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: This POLR2F antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Immunocytochemistry (ICC)

Clone: 4H8

Product Details anti-POLR2F Antibody

Purpose: RNA pol II antibody (mAb) (Clone 4H8)

Purification: Affinity Purified

Immunogen: This RNA pol II antibody was raised against a synthetic peptide YSPTSpPS corresponding to human RNA pol II. It recognizes both the phosphorylated and unphosphorylated forms of the largest subunit of RNA pol II.

Isotype: IgG1

Application Details

Application Notes: ChIP: 10 µL per ChIP ChIP-Seq: 6-20 µL each WB: 1:2,000 - 1:5,000 dilution For optimal results in Western blotting, primary antibody incubations should be performed overnight at 4 °C. The addition of 0.1 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: PBS containing 0.2 % gelatin, 0.01 % thimerosal, 0.1 % sodium azide, and 50 % glycerol. Thimerosal and sodium azide are highly toxic.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-POLR2F antibody (ABIN7938437)

Li, Kannan, Trivett, Liao, Wu, Akagi, Symer: "An antisense promoter in mouse L1 retrotransposon open reading frame-1 initiates expression of diverse fusion transcripts and limits retrotransposition." in: Nucleic acids research, Vol. 42, Issue 7, pp. 4546-62, (2014) (PubMed).

Pang, Toh, He, Teissier, Ben Khalifa, Xue, Thierry: "A functional interaction of E7 with B-Myb-MuvB complex promotes acute cooperative transcriptional activation of both S- and M-phase genes. (129 c)." in: Oncogene, Vol. 33, Issue 31, pp. 4039-49, (2014) (PubMed).

Winston, Li, Sarna: "Chronic prenatal stress epigenetically modifies spinal cord BDNF expression to induce sex-specific visceral hypersensitivity in offspring." in: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, Vol. 26, Issue 5, pp. 715-30, (2014) (PubMed).

Jha, Strahl: "An RNA polymerase II-coupled function for histone H3K36 methylation in checkpoint activation and DSB repair." in: Nature communications, Vol. 5, pp. 3965, (2014) (PubMed).

Plummer, Dan, Quinney, Hallmark, Phillips, Millar, Macpherson, Elcombe: "Identification of transcription factors and coactivators affected by dibutylphthalate interactions in fetal rat testes." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 132, Issue 2, pp. 443-57, (2013) (PubMed).

Akhrymuk, Kulemzin, Frolova: "Evasion of the innate immune response: the Old World alphavirus nsP2 protein induces rapid degradation of Rpb1, a catalytic subunit of RNA polymerase II." in: Journal of virology, Vol. 86, Issue 13, pp. 7180-91, (2012) (PubMed).

Allison, Wamsley, Kumar, Li, Gray, Hart, Jones, Mayo: "Modification of RelA by O-linked N-acetylglucosamine links glucose metabolism to NF-?B acetylation and transcription." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 42, pp. 16888-93, (2012) (PubMed).

Ellis, Shioda, Rosenthal, Coser, Shioda: "Masculine epigenetic sex marks of the CYP19A1/aromatase promoter in genetically male chicken embryonic gonads are resistant to estrogen-induced phenotypic sex conversion." in: Biology of reproduction, Vol. 87, Issue 1, pp. 23, 1-12, (2012) (PubMed).

Weishaupt, Attema: "A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP-Chip." in: Biological procedures online, Vol. 12, Issue 1, pp. 1-17, (2011) (PubMed).

Udelhoven, Leeser, Freude, Hettich, Laudes, Schnitker, Krone, Schubert: "Identification of a region in the human IRS2 promoter essential for stress induced transcription depending on SP1, NFI binding and ERK activation in HepG2 cells." in: Journal of molecular endocrinology, Vol. 44, Issue 2, pp. 99-113, (2010) (PubMed).

Udelhoven, Pasieka, Leeser, Krone, Schubert: "Neuronal insulin receptor substrate 2 (IRS2) expression is regulated by ZBP89 and SP1 binding to the IRS2 promoter." in: The Journal of endocrinology, Vol. 204, Issue 2, pp. 199-208, (2010) (PubMed).

Shirley, Rundhaug, Tian, Cullinan-Ammann, Lambertz, Conti, Fuchs-Young: "Transcriptional regulation of estrogen receptor-alpha by p53 in human breast cancer cells." in: Cancer research, Vol. 69, Issue 8, pp. 3405-14, (2009) (PubMed).

Song, Ro, Michaels, Park, McCarrey, Yan: "Many X-linked microRNAs escape meiotic sex chromosome inactivation." in: Nature genetics, Vol. 41, Issue 4, pp. 488-93, (2009) (PubMed).

Gatto, Rossi, Rossi, Kroening, Bonatti, Mallardo: "Epstein-Barr virus latent membrane protein 1 trans-activates miR-155 transcription through the NF-kappaB pathway." in: Nucleic acids research, Vol. 36, Issue 20, pp. 6608-19, (2008) (PubMed).

Raychaudhuri, Raychaudhuri, Thamotharan, Devaskar: "Histone code modifications repress glucose transporter 4 expression in the intrauterine growth-restricted offspring." in: The Journal of biological chemistry, Vol. 283, Issue 20, pp. 13611-26, (2008) (PubMed).

Leng, Butcher, Egan, Abi Abdallah, Denkers: "Toxoplasma gondii prevents chromatin remodeling initiated by TLR-triggered macrophage activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 1, pp. 489-97, (2008) (PubMed).

Coates, Lehnert, Sharma, Kindell, Gary: "Beryllium induces premature senescence in human fibroblasts." in: The Journal of pharmacology and experimental therapeutics, Vol. 322, Issue 1, pp. 70-9, (2007) (PubMed).

Romano, Delvoux, Fischer, Groothuis: "The PROGINS polymorphism of the human progesterone receptor diminishes the response to progesterone." in: Journal of molecular endocrinology, Vol. 38, Issue 1-2, pp. 331-50, (2007) (PubMed).

Kellerer, Schreiner, Stolt, Scholz, Bösl, Wegner: "Replacement of the Sox10 transcription factor by Sox8 reveals incomplete functional equivalence." in: Development (Cambridge, England), Vol. 133, Issue 15, pp. 2875-86, (2006) (PubMed).

Huffman, Geber: "Youth with disabilities and chronic illness: an international annotated bibliography." in: Pediatrician, Vol. 18, Issue 2, pp. 163-71, (1991) (PubMed).

Target Details for POLR2F

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Alternative Name: RNA Pol II

Background: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Molecular Weight: 217 kDa

Gene ID: 5430

NCBI Accession: NP_000928

Pathways: Regulatory RNA Pathways