TET2 antibody (AA 1-300)

Catalog No. ABIN7938526

This Mouse Monoclonal antibody specifically detects TET2 in WB, IP, IF and ICC. It exhibits reactivity toward Human. It has been mentioned in 2+ publications

Quick Overview for TET2 antibody (AA 1-300) (ABIN7938526)

Target: TET2 (Tet Methylcytosine Dioxygenase 2 (TET2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TET2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 21F11

Product Details anti-TET2 Antibody

Binding Specificity: AA 1-300

Purpose: Tet2 antibody (mAb) (Clone 21F11)

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a recombinant protein corresponding to amino acids 1-300 of human Tet2.

Isotype: IgG1 kappa

Application Details

Application Notes: IP: 1 - 2 µL per IP WB: 1 - 2 μg/mL dilution

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-TET2 antibody (ABIN7938526)

Wallner, Schröder, Leitão, Berulava, Haak, Beißer, Rahmann, Richter, Manke, Bönisch, Arrigoni, Fröhler, Klironomos, Chen, Rajewsky, Müller, Ebert, Lengauer, Barann, Rosenstiel, Gasparoni, Nordström et al.: "Epigenetic dynamics of monocyte-to-macrophage differentiation. ..." in: Epigenetics & chromatin, Vol. 9, pp. 33, (2016) (PubMed).

Gong, Guo, Niu, Jin, Zhang, Shi, Zhang, Li, Chen, Ma: "Epigenetic silencing of TET2 and TET3 induces an EMT-like process in melanoma." in: Oncotarget, Vol. 8, Issue 1, pp. 315-328, (2016) (PubMed).

Target Details for TET2

Target: TET2 (Tet Methylcytosine Dioxygenase 2 (TET2))

Alternative Name: Tet2

Background: Tet2 (tet methylcytosine dioxygenase 2) catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). Plays an important role in myelopoiesis. The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation and differentiation in the case of inflammation by regulating gene expression and macrophage differentiation.

Molecular Weight: 220 kDa

Gene ID: 54790

NCBI Accession: NP_001120680

Pathways: Warburg Effect