POLR2F antibody (C-Term)

Catalog No. ABIN2668536

This Rat Monoclonal antibody specifically detects POLR2F in WB, IF, ChIP and IP. It exhibits reactivity toward Human and Mouse. It has been mentioned in 3+ publications

Quick Overview for POLR2F antibody (C-Term) (ABIN2668536)

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This POLR2F antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP)

Clone: 4F8

Product Details anti-POLR2F Antibody

Binding Specificity: C-Term

Purpose: RNA pol II antibody (mAb) (Clone 4F8)

Purification: Protein G Chromatography

Immunogen: This RNA pol II antibody was raised against a synthetic peptide corresponding to human RNA pol II C-terminal heptad repeat domain.

Isotype: IgG1

Application Details

Application Notes: For RNA Pol II, we also offer an AbFlex RNA Pol II Recombinant Antibody (rAb). For details, see Catalog No. 91151.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-POLR2F antibody (ABIN2668536)

Catania, Pidoux, Allshire: "Sequence features and transcriptional stalling within centromere DNA promote establishment of CENP-A chromatin." in: PLoS genetics, Vol. 11, Issue 3, pp. e1004986, (2015) (PubMed).

Dahiya, Liu, Nonnemacher, Dampier, Wigdahl: "CCAAT enhancer binding protein and nuclear factor of activated T cells regulate HIV-1 LTR via a novel conserved downstream site in cells of the monocyte-macrophage lineage." in: PLoS ONE, Vol. 9, Issue 2, pp. e88116, (2014) (PubMed).

Chapman, Heidemann, Albert, Mailhammer, Flatley, Meisterernst, Kremmer, Eick: "Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7." in: Science (New York, N.Y.), Vol. 318, Issue 5857, pp. 1780-2, (2007) (PubMed).

Target Details for POLR2F

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Alternative Name: RNA Pol II

Background: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Molecular Weight: 240 kDa

Gene ID: 5430

NCBI Accession: NP_000928

Pathways: Regulatory RNA Pathways