CHD5 antibody (C-Term)

Catalog No. ABIN2668553

The Rat Monoclonal anti-CHD5 antibody (Clone 5A10) (ABIN2668553) specifically detects CHD5 in WB, ICC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for CHD5 antibody (C-Term) (ABIN2668553)

Target: CHD5 (Chromodomain Helicase DNA Binding Protein 5 (CHD5))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CHD5 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 5A10

Product Details anti-CHD5 Antibody

Binding Specificity: C-Term

Purpose: Chd5 antibody (mAb) (Clone 5A10)

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a peptide within the C-terminal region of mouse Chd5.

Isotype: IgG2a

Application Details

Application Notes: ICC/IF: 2 - 5 μg/mL dilution WB*: 1 - 2 μg/mL dilution The addition of 0.1 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for CHD5

Target: CHD5 (Chromodomain Helicase DNA Binding Protein 5 (CHD5))

Alternative Name: Chd5

Background: Chd5 (Chromodomain Helicase DNA Binding Protein 5) is member of the CHD subfamily of chromatin remodeling SWI/SNF proteins, containing chromodomains and a SNF2-like ATPase/helicase domain, that play a role in chromatin remodeling and gene transcription. Chd5 is also a potential tumor suppressor that may play a role in neuronal development.

Molecular Weight: 260 kDa

Gene ID: 269610

NCBI Accession: NP_001074845