DDIT3 antibody

Catalog No. ABIN2668558

There are 2+ publications for this product available. The Mouse Monoclonal anti-DDIT3 antibody is suitable to detect DDIT3 in samples from Mouse. It has been validated for WB and IP.

Quick Overview for DDIT3 antibody (ABIN2668558)

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This DDIT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 9C8

Product Details anti-DDIT3 Antibody

Purpose: Ddit3 antibody (mAb) (Clone 9C8)

Purification: Protein G Chromatography

Immunogen: This antibody was raised against full-length recombinant mouse Ddit3 protein.

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-DDIT3 antibody (ABIN2668558)

Kuroda, Wang, Sok, Yin, Chung, Giannotti, Jacobs, Fitz, Murtha-Riel, Turner, Ron: "Induction of a secreted protein by the myxoid liposarcoma oncogene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 9, pp. 5025-30, (1999) (PubMed).

Wang, Kuroda, Sok, Batchvarova, Kimmel, Chung, Zinszner, Ron: "Identification of novel stress-induced genes downstream of chop." in: The EMBO journal, Vol. 17, Issue 13, pp. 3619-30, (1998) (PubMed).

Target Details for DDIT3

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Alternative Name: Ddit3

Background: Ddit3 (DNA-damage-inducible transcript 3, CHOP) is a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis.

Molecular Weight: 29 kDa

Gene ID: 13198

NCBI Accession: NP_031863

Pathways: Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis