RNF2 antibody

Catalog No. ABIN2668629

This Mouse Monoclonal antibody specifically detects RNF2 in WB, IF, IP, ChIP, ICC and ChIP-seq. It exhibits reactivity toward Human, Mouse and Monkey. It has been mentioned in 4+ publications

Quick Overview for RNF2 antibody (ABIN2668629)

Target: RNF2 (Ring Finger Protein 2 (RNF2))

Reactivity: Human, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This RNF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq)

Clone: 3-3

Product Details anti-RNF2 Antibody

Purpose: Ring1B antibody (mAb) (Clone 3-3)

Purification: Protein G Chromatography

Immunogen: This Ring1B antibody was raised against the recombinant full-length mouse protein.

Isotype: IgG2b

Application Details

Application Notes: ChIP-Seq: 4 µL each

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-RNF2 antibody (ABIN2668629)

Jacinto, Benner, Hetzer: "The nucleoporin Nup153 regulates embryonic stem cell pluripotency through gene silencing." in: Genes & development, Vol. 29, Issue 12, pp. 1224-38, (2015) (PubMed).

Basu, Wilkinson, Colavita, Fennelly, Atchison: "YY1 DNA binding and interaction with YAF2 is essential for Polycomb recruitment." in: Nucleic acids research, Vol. 42, Issue 4, pp. 2208-23, (2014) (PubMed).

Lin, Shen, Li, Tang, Gu, Chen, Hu, Rice, Lu, Wu: "Proteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program." in: The Journal of biological chemistry, Vol. 288, Issue 9, pp. 6238-47, (2013) (PubMed).

Lavial, Bessonnard, Ohnishi, Tsumura, Chandrashekran, Fenwick, Tomaz, Hosokawa, Nakayama, Chambers, Hiiragi, Chazaud, Azuara: "Bmi1 facilitates primitive endoderm formation by stabilizing Gata6 during early mouse development." in: Genes & development, Vol. 26, Issue 13, pp. 1445-58, (2012) (PubMed).

Target Details for RNF2

Target: RNF2 (Ring Finger Protein 2 (RNF2))

Alternative Name: Ring1B

Background: Ring1B (also known as ring finger protein 2, RING2 or RNF2) is an essential component of the Polycomb group (PcG) multiprotein PRC1 complex. The PRC1 complex is important for the transcriptional repression of genes involved in development and cell proliferation, such as the Hox genes. Ring1B also acts as an E3 ubiquitin ligase on histone H2A of the PRC1 complex.

Molecular Weight: 40 kDa

Gene ID: 6045

NCBI Accession: NP_009143