HDAC3 antibody (AA 411-428)

Catalog No. ABIN2668680

This Mouse Monoclonal antibody specifically detects HDAC3 in WB and IP. It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for HDAC3 antibody (AA 411-428) (ABIN2668680)

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HDAC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 3G6

Product Details anti-HDAC3 Antibody

Binding Specificity: AA 411-428

Purpose: HDAC3 antibody (mAb) (Clone 3G6)

Purification: Protein G Chromatography

Immunogen: This HDAC3 antibody was raised against a synthetic peptide corresponding to amino acids 411-428 of human HDAC3.

Isotype: IgG

Application Details

Application Notes: This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91199.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-HDAC3 antibody (ABIN2668680)

Mueller, Breuer, Schmitt, Walter, Evert, Wüllner: "CK2-dependent phosphorylation determines cellular localization and stability of ataxin-3." in: Human molecular genetics, Vol. 18, Issue 17, pp. 3334-43, (2009) (PubMed).

Target Details for HDAC3

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Alternative Name: HDAC3

Background: HDAC3 (Histone Deacetylase 3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). By forming multi-protein complexes with the co-repressors SMRT and N-CoR, HDAC3 regulates the transcription of a plethora of genes. A growing list of non-histone proteins extends the role of HDAC3 beyond transcriptional repression. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.

Molecular Weight: 50 kDa

Gene ID: 8841

NCBI Accession: NP_003874

Pathways: Neurotrophin Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development