SUV39H1 antibody (AA 1-126)

Catalog No. ABIN2668694

Cited in 3+ publications. The Mouse Monoclonal anti-SUV39H1 antibody (Clone MG44) (ABIN2668694) specifically detects SUV39H1 in WB, IP, ChIP, IF and ICC. The antibody is reactive with Human and Mouse samples.

Quick Overview for SUV39H1 antibody (AA 1-126) (ABIN2668694)

Target: SUV39H1 (Suppressor of Variegation 3-9 Homolog 1 (Drosophila) (SUV39H1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SUV39H1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: MG44

Product Details anti-SUV39H1 Antibody

Binding Specificity: AA 1-126

Purpose: SUV39H1 antibody (mAb) (Clone MG44)

Purification: Protein G Chromatography

Immunogen: This SUV39H1 antibody was raised against a recombinant protein corresponding to amino acids 1-126 of mouse SUV39H1.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-SUV39H1 antibody (ABIN2668694)

Lee, Hung, Hung, Shieh: "Epidermal growth factor receptor regulates beta-catenin location, stability, and transcriptional activity in oral cancer." in: Molecular cancer, Vol. 9, pp. 64, (2010) (PubMed).

Zhou, Wang, Chen, Carmichael: "On the mechanism of induction of heterochromatin by the RNA-binding protein vigilin." in: RNA (New York, N.Y.), Vol. 14, Issue 9, pp. 1773-81, (2008) (PubMed).

Terada: "Aurora-B/AIM-1 regulates the dynamic behavior of HP1alpha at the G2-M transition." in: Molecular biology of the cell, Vol. 17, Issue 7, pp. 3232-41, (2006) (PubMed).

Target Details for SUV39H1

Target: SUV39H1 (Suppressor of Variegation 3-9 Homolog 1 (Drosophila) (SUV39H1))

Alternative Name: SUV39H1

Background: SUV39H1 is one of two homologues of the Drosophila Suppressor-of-variegation 3(9) protein (aka Su(var)3-9). SUV39H1 is a SET domain-containing histone methyltransferase specific for lysine 9 of histone H3. Lysine 9 methylation of histone H3 is found at regions of the genome that are transcriptionally silenced, such as centromeres, repetitive elements and inactive genes. H3 lysine 9 methylation is a hallmark of heterochromatin, regions of the genome that are in a tightly compacted state and relatively devoid of gene expression. HP1 proteins recognize and bind lysine 9 methylation and this contributes to maintaining heterochromatin in its compact and inactive state. SUV39H1 also contains a chromodomain, which may bind to lysine 9 methylation and contribute to the regional spreading of lysine 9 methylation and the establishment of heterochromatin over broader domains of the genome.

Molecular Weight: 50 kDa

Gene ID: 6839

NCBI Accession: NP_035644