NAPSA antibody

Catalog No. ABIN2668697

Cited in 1 publication. The Rabbit Polyclonal anti-NAPSA antibody (ABIN2668697) specifically detects NAPSA in WB and IF. The antibody is reactive with Drosophila melanogaster samples.

Quick Overview for NAPSA antibody (ABIN2668697)

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Reactivity: Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAPSA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-NAPSA Antibody

Purpose: Nap1 antibody (pAb)

Purification: Affinity Purified

Immunogen: This Nap1 antibody was raised against a His-Tagged full-length protein corresponding to Drosophila Nap1.

Isotype: IgG

Application Details

Application Notes: WB: 1:1,000 - 1:5,000 dilution The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.1 % sodium azide, 0.2 mg/mL BSA and 35 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-NAPSA antibody (ABIN2668697)

Kimura: "The Nap family proteins, CG5017/Hanabi and Nap1, are essential for Drosophila spermiogenesis." in: FEBS letters, Vol. 587, Issue 7, pp. 922-9, (2013) (PubMed).

Target Details for NAPSA

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Alternative Name: Nap1

Background: Nap1 (Nucleosome assembly protein 1) is a member of the nucleosome assembly protein (NAP) family. Nap1 serves as a chaperone for Histone H2A-Histone H2B dimers, and participates in DNA replication and may play a role in modulating chromatin formation and contribute to the regulation of cell proliferation. Nap1 binds the HIV Rev protein, stimulating its RNA export function, while causing it to localize away from the nucleolus.

Molecular Weight: 51 kDa

Gene ID: 37798

NCBI Accession: NP_477128

Pathways: Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis