Retinoic Acid Receptor alpha antibody (AA 420-443)

Catalog No. ABIN2668711

There are 2+ publications for this product available. The Mouse Monoclonal anti-Retinoic Acid Receptor alpha antibody is suitable to detect Retinoic Acid Receptor alpha in samples from Human and Mouse. It has been validated for WB, IHC and SSA.

Quick Overview for Retinoic Acid Receptor alpha antibody (AA 420-443) (ABIN2668711)

Target: Retinoic Acid Receptor alpha (RARA) (Retinoic Acid Receptor, alpha (RARA))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Retinoic Acid Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Supershift Assay (SSA)

Clone: 9alpha-9A6

Product Details anti-Retinoic Acid Receptor alpha Antibody

Binding Specificity: AA 420-443

Purpose: RAR-alpha antibody (mAb) (Clone 9a-9A6)

Purification: Protein A Chromatography

Immunogen: This RAR-a antibody was raised against a recombinant protein corresponding to amino acids 420-443 (the F region) of human RARa.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.71 μg/μL

Buffer: Purified IgG in 60 mM Sodium Citrate, 105 mM Tris-HCl, 30 % glycerol, and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Retinoic Acid Receptor alpha antibody (ABIN2668711)

Quere, Baudet, Cassinat, Bertrand, Marti, Manchon, Piquemal, Chomienne, Commes: "Pharmacogenomic analysis of acute promyelocytic leukemia cells highlights CYP26 cytochrome metabolism in differential all-trans retinoic acid sensitivity." in: Blood, Vol. 109, Issue 10, pp. 4450-60, (2007) (PubMed).

Reichrath, Mittmann, Kamradt, Müller: "Expression of retinoid-X receptors (-alpha,-beta,-gamma) and retinoic acid receptors (-alpha,-beta,-gamma) in normal human skin: an immunohistological evaluation." in: The Histochemical journal, Vol. 29, Issue 2, pp. 127-33, (1997) (PubMed).

Target Details for Retinoic Acid Receptor alpha

Target: Retinoic Acid Receptor alpha (RARA) (Retinoic Acid Receptor, alpha (RARA))

Alternative Name: RAR-alpha

Background: Retinoic acid receptors (RAR) are nuclear receptors that are activated by both all-trans retinoic acid and 9-cis retinoic acid. There are three retinoic acid receptors (RAR), RAR-a, RAR-b and RAR-g. RAR-a (Nuclear receptor subfamily 1 group B member 1, NR1B1) interacts with NCoA3, NCoA6, NCoA7 and MLL to activate transcription of target genes. Chromosomal aberrations of RAR-a are sometimes involved in Promyelocytic leukemia.

Molecular Weight: 52 kDa

Gene ID: 5914

NCBI Accession: NP_001138773

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, S100 Proteins