Retinoic Acid Receptor gamma antibody (AA 428-441)

Catalog No. ABIN2668712

Cited in 2+ publications. The Mouse Monoclonal anti-Retinoic Acid Receptor gamma antibody (Clone 4gamma-7A11) (ABIN2668712) specifically detects Retinoic Acid Receptor gamma in WB, IHC and SSA. The antibody is reactive with Human samples.

Quick Overview for Retinoic Acid Receptor gamma antibody (AA 428-441) (ABIN2668712)

Target: Retinoic Acid Receptor gamma (RARG) (Retinoic Acid Receptor, gamma (RARG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Retinoic Acid Receptor gamma antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Supershift Assay (SSA)

Clone: 4gamma-7A11

Product Details anti-Retinoic Acid Receptor gamma Antibody

Binding Specificity: AA 428-441

Purpose: RAR-gamma antibody (mAb) (Clone 4g-7A11)

Purification: Protein G Chromatography

Immunogen: This RAR-g antibody was raised against a recombinant protein corresponding to amino acids 428-441 (F-region) of human RARg.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Retinoic Acid Receptor gamma antibody (ABIN2668712)

Hoftijzer, Liu, Morreau, van Wezel, Pereira, Corssmit, Romijn, Smit: "Retinoic acid receptor and retinoid X receptor subtype expression for the differential diagnosis of thyroid neoplasms." in: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 160, Issue 4, pp. 631-8, (2009) (PubMed).

Reichrath, Mittmann, Kamradt, Müller: "Expression of retinoid-X receptors (-alpha,-beta,-gamma) and retinoic acid receptors (-alpha,-beta,-gamma) in normal human skin: an immunohistological evaluation." in: The Histochemical journal, Vol. 29, Issue 2, pp. 127-33, (1997) (PubMed).

Target Details for Retinoic Acid Receptor gamma

Target: Retinoic Acid Receptor gamma (RARG) (Retinoic Acid Receptor, gamma (RARG))

Alternative Name: RAR-gamma

Background: Retinoic acid receptors (RAR) are nuclear receptors that are activated by both all-trans retinoic acid and 9-cis retinoic acid. There are three retinoic acid receptors (RAR), RAR-a, RAR-b and RAR-g. RAR-g has two splice isoforms. RAR-g heterodimerizes with RXR and in the absence of ligand, the RAR/RXR dimer binds to hormone response elements complexed with co-repressor protein. Binding of agonist ligands to RAR results in dissociation of co-repressor and recruitment of coactivator protein which in turn promotes transcription of the downstream target gene into mRNA and eventually protein.

Molecular Weight: 52 kDa

Gene ID: 5916

NCBI Accession: NP_000957

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Cell Size