WWTR1 antibody (C-Term)

Catalog No. ABIN2668742

The Rabbit Polyclonal anti-WWTR1 antibody (ABIN2668742) specifically detects WWTR1 in WB. The antibody is reactive with Human samples.

Quick Overview for WWTR1 antibody (C-Term) (ABIN2668742)

Target: WWTR1 (WW Domain Containing Transcription Regulator 1 (WWTR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WWTR1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-WWTR1 Antibody

Binding Specificity: C-Term

Purpose: TAZ / WWTR1 antibody (pAb)

Purification: Affinity Purified

Immunogen: TAZ / WWTR1 antibody was raised against a peptide corresponding to the C-terminal region of human TAZ / WWTR1.

Isotype: IgG

Application Details

Application Notes: WB: 1:500 - 1:1,000 dilution

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.15 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for WWTR1

Target: WWTR1 (WW Domain Containing Transcription Regulator 1 (WWTR1))

Alternative Name: TAZ / WWTR1

Background: The TAZ / WWTR1 (Transcriptional coactivator with PDZ-binding motif, WW domain-containing transcription regulator protein 1) gene encodes a protein that is a transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.

Molecular Weight: 55 kDa

Gene ID: 25937

NCBI Accession: NP_056287

Pathways: Regulation of Lipid Metabolism by PPARalpha