CARM1 antibody (C-Term)

Catalog No. ABIN2668761

This Rabbit Polyclonal antibody specifically detects CARM1 in WB, IHC, IP and ChIP. It exhibits reactivity toward Human, Rat and Mouse.It has been mentioned in 1 publication

Quick Overview for CARM1 antibody (C-Term) (ABIN2668761)

Target: CARM1 (Coactivator-Associated Arginine Methyltransferase 1 (CARM1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CARM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Product Details anti-CARM1 Antibody

Binding Specificity: C-Term

Purpose: CARM1 antibody (pAb)

Purification: Unpurified

Immunogen: This CARM1 antibody was raised against a peptide corresponding to the C-terminus of mouse CARM1.

Application Details

Application Notes: WB: 1:250 - 1:1,000 dilution The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-CARM1 antibody (ABIN2668761)

Mann, Cortez, Vadlamudi: "PELP1 oncogenic functions involve CARM1 regulation." in: Carcinogenesis, Vol. 34, Issue 7, pp. 1468-75, (2013) (PubMed).

Target Details for CARM1

Target: CARM1 (Coactivator-Associated Arginine Methyltransferase 1 (CARM1))

Alternative Name: CARM1

Background: CARM1 (Coactivator-associated arginine methyltransferase 1 or CARM 1) is a protein arginine N-methyltransferase that catalyzes the mono- and asymmetric dimethylation of arginine residues in its substrate proteins. It serves as a coactivator of transcription and also plays a role in nuclear receptors. CARM1 methylates histone H3 at Arg2, Arg17 and Arg26, it also methylates a number of non-histone substrates involved in the regulation and mechanism of gene expression.

Molecular Weight: 60 kDa

Gene ID: 10498

NCBI Accession: NP_067506

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of fat Cell Differentiation