SRF antibody

Catalog No. ABIN2668813

There are 2+ publications for this product available. The Rat Monoclonal anti-SRF antibody is suitable to detect SRF in samples from Human and Mouse. It has been validated for WB, IHC, IF, ChIP and ChIP-seq.

Quick Overview for SRF antibody (ABIN2668813)

Target: SRF (Serum Response Factor (SRF))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This SRF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Clone: 2C5

Product Details anti-SRF Antibody

Purpose: SRF antibody (mAb) (Clone 2C5)

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a full-length protein corresponding to mouse SRF.

Isotype: IgG2a

Application Details

Application Notes: ChIP: 5 - 10 µL per ChIP ChIP-Seq: 5 - 10 µL each ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-SRF antibody (ABIN2668813)

Werfel, Jungmann, Lehmann, Ksienzyk, Bekeredjian, Kaya, Leuchs, Nordheim, Backs, Engelhardt, Katus, Müller: "Rapid and highly efficient inducible cardiac gene knockout in adult mice using AAV-mediated expression of Cre recombinase." in: Cardiovascular research, Vol. 104, Issue 1, pp. 15-23, (2014) (PubMed).

Koegel, von Tobel, Schäfer, Alberti, Kremmer, Mauch, Hohl, Wang, Beer, Bloch, Nordheim, Werner: "Loss of serum response factor in keratinocytes results in hyperproliferative skin disease in mice." in: The Journal of clinical investigation, Vol. 119, Issue 4, pp. 899-910, (2009) (PubMed).

Target Details for SRF

Target: SRF (Serum Response Factor (SRF))

Alternative Name: SRF

Background: SRF (serum response factor) is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways, for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).

Molecular Weight: 67 kDa

Gene ID: 20807

NCBI Accession: NP_065239