IKZF1 antibody (N-Term)

Catalog No. ABIN2668828

There is 1 publication for this product available. The Mouse Monoclonal anti-IKZF1 antibody is suitable to detect IKZF1 in samples from Mouse. It has been validated for WB, ChIP and ChIP-seq.

Quick Overview for IKZF1 antibody (N-Term) (ABIN2668828)

Target: IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IKZF1 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Clone: 2A9

Product Details anti-IKZF1 Antibody

Binding Specificity: N-Term

Purpose: Ikaros antibody (mAb) (Clone 2A9)

Purification: Protein G Chromatography

Immunogen: This Ikaros antibody was raised against a recombinant protein corresponding to the N-terminal half of mouse Ikaros.

Isotype: IgG

Application Details

Application Notes: ChIP: 4 μg per ChIP ChIP-Seq: 4 μg each WB: 0.5 - 2 μg/mL dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-IKZF1 antibody (ABIN2668828)

Yeung, Chung, Guess, Bell, Leinwand: "Myh7b/miR-499 gene expression is transcriptionally regulated by MRFs and Eos." in: Nucleic acids research, Vol. 40, Issue 15, pp. 7303-18, (2012) (PubMed).

Target Details for IKZF1

Target: IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

Alternative Name: Ikaros

Background: Ikaros is a transcription factor that plays a critical role in the control of lymphohematopoiesis and immune regulation. All Ikaros isoforms share a common C-terminal domain that contains a transcription activation motif and two zinc finger motifs required for hetero- and homo-dimerization among the Ikaros isoforms, and for interactions with other proteins. Ikaros is abundantly expressed in pituitary mammosomatotrophs, where it deacetylates Histone H3 sites on the proximal growth hormone (GH) promoter to silence gene expression.

Molecular Weight: 62 kDa

Gene ID: 22778

NCBI Accession: NM_001025597

Pathways: Production of Molecular Mediator of Immune Response