Histone H4 antibody (meLys20)

Catalog No. ABIN2668854

This Mouse Monoclonal antibody specifically detects Histone H4 in WB, IF, ICC, ChIP, DB and ChIP-seq. It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for Histone H4 antibody (meLys20) (ABIN2668854)

Target: Histone H4 (HISTH4)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Clone: 5E10-D8

Product Details anti-Histone H4 Antibody

Binding Specificity: meLys20

Purpose: Histone H4K20me1 antibody (mAb) (Clone 5E10-D8)

Purification: Protein A Chromatography

Immunogen: This Histone H4 monomethyl Lys20 was raised against a synthetic peptide containing monomethyl Lys20 of Histone H4.

Isotype: IgG1 kappa

Application Details

Application Notes: ChIP: 5 - 10 μg per ChIP ChIP-Seq: 5 - 10 μg each ICC/IF: 1 μg/mL dilution WB: 0.5 - 2 μg/mL dilution DB: 1 μg/mL dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here. For Histone H4K20me1, we also offer AbFlex Histone H4K20me1 Recombinant Antibody (rAb). For details, see Catalog No. 91291.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: PBS containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone H4 antibody (ABIN2668854)

Brasa, Mueller, Jacquemont, Hahne, Rozenberg, Peters, He, McCormack, Gasparini, Chibout, Grenet, Moggs, Gomez-Mancilla, Terranova: "Reciprocal changes in DNA methylation and hydroxymethylation and a broad repressive epigenetic switch characterize FMR1 transcriptional silencing in fragile X syndrome." in: Clinical epigenetics, Vol. 8, pp. 15, (2016) (PubMed).

Target Details for Histone H4

Target: Histone H4 (HISTH4)

Alternative Name: Histone H4

Background: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression. Monomethylation of histone H4 at lysine 20 is detected on the inactive X chromosome in female mammals and implicated in chromosome condensation.

Molecular Weight: 8 kDa

Gene ID: 121504

NCBI Accession: NP_778224