Histone H4 antibody (H4K20me3)

Catalog No. ABIN2668855

There are 3+ publications for this product available. The Mouse Monoclonal anti-Histone H4 antibody is suitable to detect Histone H4 in samples from Human and Mouse. It has been validated for WB, ICC, ChIP and DB.

Quick Overview for Histone H4 antibody (H4K20me3) (ABIN2668855)

Target: Histone H4 (HISTH4)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB)

Clone: 6F8-D9

Product Details anti-Histone H4 Antibody

Binding Specificity: H4K20me3

Purpose: Histone H4K20me3 antibody (mAb) (Clone 6F8-D9)

Purification: Protein G Chromatography

Immunogen: This Histone H4 trimethyl Lys20 antibody (mAb) was raised against a peptide containing trimethyl Lys20 of Histone H4.

Isotype: IgG

Application Details

Application Notes: ChIP: 5 - 10 μg per ChIP WB*: 1 - 2 μg/mL dilution DB: 0.2 μg/mL dilution Slight reactivity towards histone H4 dimethyl Lys20 might be observed under certain conditions. Individual optimization may be required. *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer can be used to aid in detection by Western blot. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 0.035 % sodium azide and 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone H4 antibody (ABIN2668855)

Gonzalez-Vasconcellos, Schneider, Anastasov, Alonso-Rodriguez, Sanli-Bonazzi, Fernández, Atkinson: "The Rb1 tumour suppressor gene modifies telomeric chromatin architecture by regulating TERRA expression." in: Scientific reports, Vol. 7, pp. 42056, (2017) (PubMed).

Dal-Pra, Hodgkinson, Mirotsou, Kirste, Dzau: "Demethylation of H3K27 Is Essential for the Induction of Direct Cardiac Reprogramming by miR Combo." in: Circulation research, Vol. 120, Issue 9, pp. 1403-1413, (2017) (PubMed).

Nelson, Jaber-Hijazi, Cole, Robertson, Pawlikowski, Norris, Criscione, Pchelintsev, Piscitello, Stong, Rai, McBryan, Otte, Nixon, Clark, Riethman, Wu, Schotta, Garcia, Neretti, Baird, Berger, Adams: "Mapping H4K20me3 onto the chromatin landscape of senescent cells indicates a function in control of cell senescence and tumor suppression through preservation of genetic and epigenetic stability." in: Genome biology, Vol. 17, Issue 1, pp. 158, (2016) (PubMed).

Target Details for Histone H4

Target: Histone H4 (HISTH4)

Alternative Name: Histone H4

Background: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression.

Molecular Weight: 8 kDa

Gene ID: 121504

NCBI Accession: NP_778224