Histone H4 antibody (H4K20me2)

Catalog No. ABIN2668862

The Mouse Monoclonal anti-Histone H4 antibody (Clone MABI 0422) (ABIN2668862) specifically detects Histone H4 in WB and DB. The antibody is reactive with Human samples.

Quick Overview for Histone H4 antibody (H4K20me2) (ABIN2668862)

Target: Histone H4 (HISTH4)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB)

Clone: MABI 0422

Product Details anti-Histone H4 Antibody

Binding Specificity: H4K20me2

Purpose: Histone H4K20me2 antibody (mAb) (Clone MABI 0422)

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a synthetic peptide containing dimethyl-lysine 20 of human Histone H4.

Isotype: IgG1

Application Details

Application Notes: WB*: 0.5 - 2 μg/mL dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Concentration: 0.59 μg/μL

Buffer: Purified IgG in PBS, 0.3 M NaCl with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for Histone H4

Target: Histone H4 (HISTH4)

Alternative Name: Histone H4

Background: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression.

Molecular Weight: 8 kDa

Gene ID: 121504

NCBI Accession: NP_778224