Histone H4 antibody (acetylated)

Catalog No. ABIN2668866

This Rabbit Polyclonal antibody specifically detects Histone H4 in WB, IHC, IF, ICC, ChIP, DB and ChIP-seq. It exhibits reactivity toward Human and Mouse. It has been mentioned in 2+ publications

Quick Overview for Histone H4 antibody (acetylated) (ABIN2668866)

Target: Histone H4 (HISTH4)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-Histone H4 Antibody

Binding Specificity: acetylated

Purpose: Histone H4ac (pan-acetyl) antibody (pAb)

Purification: Protein A Chromatography

Immunogen: This Histone H4 pan-acetyl antibody was raised against a peptide containing the amino terminal region of Tetrahymena hv1, an H2A variant with homology to mammalian histone H4. The antibody recognizes acetylated histone H4, but not H2A, in HeLa extracts.

Isotype: IgG

Application Details

Application Notes: ChIP: 5 - 10 μg per ChIP ChIP-Seq: 5 - 10 μg each ICC/IF: 1 - 2 μg/mL dilution IHC (FFPE): 1:1000 dilution WB: 1 - 2 μg/mL dilution

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone H4 antibody (ABIN2668866)

Ito, Egusa, Maeda, Numata, Nakano, Nishiyama, Tsuboi: "IL-33 promotes MHC class II expression in murine mast cells." in: Immunity, inflammation and disease, Vol. 3, Issue 3, pp. 196-208, (2015) (PubMed).

Soukup, Chiang, Bok, Reyes-Dominguez, Oakley, Wang, Strauss, Keller: "Overexpression of the Aspergillus nidulans histone 4 acetyltransferase EsaA increases activation of secondary metabolite production." in: Molecular microbiology, Vol. 86, Issue 2, pp. 314-30, (2012) (PubMed).

Target Details for Histone H4

Target: Histone H4 (HISTH4)

Alternative Name: Histone H4

Background: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H4 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as Hat1 or Gcn5. Acetylation of histones is often associated with transcriptional activation.

Molecular Weight: 8 kDa

Gene ID: 121504

NCBI Accession: XP_001016593