ZBTB16 antibody

Catalog No. ABIN2668927

There are 3+ publications for this product available. The Mouse Monoclonal anti-ZBTB16 antibody is suitable to detect ZBTB16 in samples from Human and Mouse. It has been validated for WB, IF, ICC, IP and ChIP.

Quick Overview for ZBTB16 antibody (ABIN2668927)

Target: ZBTB16 (Zinc Finger and BTB Domain Containing 16 (ZBTB16))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZBTB16 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Clone: 2A9

Product Details anti-ZBTB16 Antibody

Purpose: PLZF antibody (mAb) (Clone 2A9)

Purification: Protein G Chromatography

Immunogen: This PLZF antibody was raised against a recombinant protein corresponding to full-length mouse PLZF with a GST-Tag.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: PBS pH 7.5 containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-ZBTB16 antibody (ABIN2668927)

Felli, Errico, Pedini, Petrini, Puglisi, Bellenghi, Boe, Felicetti, Mattia, De Feo, Bottero, Tripodo, Carè: "AP2α controls the dynamic balance between miR-126&126* and miR-221&222 during melanoma progression." in: Oncogene, Vol. 35, Issue 23, pp. 3016-26, (2016) (PubMed).

Chapman, Medrano, Chaudhary, Hamra: "NRG1 and KITL Signal Downstream of Retinoic Acid in the Germline to Support Soma-Free Syncytial Growth of Differentiating Spermatogonia." in: Cell death discovery, Vol. 1, (2015) (PubMed).

Koken, Reid, Quignon, Chelbi-Alix, Davies, Kabarowski, Zhu, Dong, Chen, Chen, Tan, Licht, Waxman, de Thé, Zelent: "Leukemia-associated retinoic acid receptor alpha fusion partners, PML and PLZF, heterodimerize and colocalize to nuclear bodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 19, pp. 10255-60, (1997) (PubMed).

Target Details for ZBTB16

Target: ZBTB16 (Zinc Finger and BTB Domain Containing 16 (ZBTB16))

Alternative Name: PLZF

Background: PLZF (Promyelocytic Leukemia Zinc-Finger protein) is a member of the POK (POZ and Krüppel) family of transcriptional repressors. PLZF is expressed in early, but not differentiated, hematopoietic cells, and is required for spermatogonial stem cell maintenance. In addition, PLZF regulates genes involved in cellular proliferation and differentiation (cyclin A2, Myc and Hox genes) and targeted deletion of PLZF in mice results in limb and axial skeleton and limb deformations.

Molecular Weight: 90 kDa

Gene ID: 235320

NCBI Accession: NP_001028496

Pathways: Positive Regulation of fat Cell Differentiation