PROX1 antibody (AA 547-737)

Catalog No. ABIN2668929

This Mouse Monoclonal antibody specifically detects PROX1 in IHC, IF and ICC. It exhibits reactivity toward Human. It has been mentioned in 2+ publications

Quick Overview for PROX1 antibody (AA 547-737) (ABIN2668929)

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PROX1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 5G10

Product Details anti-PROX1 Antibody

Binding Specificity: AA 547-737

Purpose: Prox1 antibody (mAb) (Clone 5G10)

Purification: Protein G Chromatography

Immunogen: This Prox1 antibody was raised against a recombinant protein corresponding to amino acids 547-737 of human Prox1.

Isotype: IgG1

Application Details

Application Notes: For a Prox1 antibody that has been validated for use in Western blot, see Catalog No. 61091.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-PROX1 antibody (ABIN2668929)

Digilio, Yap, Winckler et al.: "Ctip2-, Satb2-, Prox1-, and GAD65-Expressing Neurons in Rat Cultures: Preponderance of Single- and Double-Positive Cells, and Cell Type-Specific Expression of Neuron-Specific Gene Family Members, ..." in: PLoS ONE, Vol. 10, Issue 10, pp. e0140010, (2015) (PubMed).

Chen, Patel, Cain, Duncan: "Production of monoclonal antibodies against Prox1." in: Hybridoma (2005), Vol. 25, Issue 1, pp. 27-33, (2006) (PubMed).

Target Details for PROX1

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Alternative Name: Prox1

Background: Prox1 (Prospero-like homeobox protein 1) is a sequence-specific DNA binding transcription factor. Prox1 controls lymphatic endothelial and retinal progenitor cell differentiation, as well as development of the liver and lens of the eye. Required for lymphatic endothelial cells specification and the formation of lymphatic vessels.

Molecular Weight: 90 kDa

Gene ID: 5629

NCBI Accession: NP_002754

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation