SUZ12 antibody

Catalog No. ABIN2668948

There are 2+ publications for this product available. The Mouse Monoclonal anti-SUZ12 antibody is suitable to detect SUZ12 in samples from Human. It has been validated for IHC, IP and ChIP.

Quick Overview for SUZ12 antibody (ABIN2668948)

Target: SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SUZ12 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Clone: 2AO9

Product Details anti-SUZ12 Antibody

Purpose: Suz12 antibody (mAb) (Clone 2AO9)

Purification: Protein G Chromatography

Immunogen: This Suz12 antibody was raised against a recombinant protein corresponding to full-length human Suz12.

Isotype: IgG2b

Application Details

Application Notes: ChIP: 2.5 - 10 μg per ChIP This antibody has been published, but has not been validated by Active Motif, for Western blot. For an antibody validated for use in Western blotting, see Catalog No. 39357. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91163.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-SUZ12 antibody (ABIN2668948)

Pasini, Hansen, Christensen, Agger, Cloos, Helin: "Coordinated regulation of transcriptional repression by the RBP2 H3K4 demethylase and Polycomb-Repressive Complex 2." in: Genes & development, Vol. 22, Issue 10, pp. 1345-55, (2008) (PubMed).

Herranz, Pasini, Díaz, Francí, Gutierrez, Dave, Escrivà, Hernandez-Muñoz, Di Croce, Helin, García de Herreros, Peiró: "Polycomb complex 2 is required for E-cadherin repression by the Snail1 transcription factor." in: Molecular and cellular biology, Vol. 28, Issue 15, pp. 4772-81, (2008) (PubMed).

Target Details for SUZ12

Target: SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))

Alternative Name: Suz12

Background: Suz12 (Suppressor-of-Zeste 12 protein, also designated as ChET9, JJAZ1 protein) is a component of the Polycomb group complexes 2, 3 and 4 (PRC2, PRC3 and PRC4), which also contain the histone methyltransferase EZH2, EED (Embryonic Ectoderm Development protein) protein and the histone-binding proteins RbAP46 and RbAP48. PRC2 is responsible for di- and trimethylation of histone 3 at lysine 27 (Histone H3K27me2 and Histone H3K27me3), which leads to transcriptional repression of the target genes. Suz12 is a nuclear protein belonging to the VEFS family. Suz12 contains a zinc-finger binding motif and a VEFS box, which is required for the interaction between Suz12 and EZH2.

Molecular Weight: 95 kDa

Gene ID: 23512

NCBI Accession: NP_056170