DNMT3B antibody

Catalog No. ABIN2668953

This Rat Monoclonal antibody specifically detects DNMT3B in WB. It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for DNMT3B antibody (ABIN2668953)

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This DNMT3B antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 2C1

Product Details anti-DNMT3B Antibody

Purpose: DNMT3B antibody (mAb) (Clone 2C1)

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a peptide corresponding to amino acid residue(s) 383-396 of human DNMT3b.

Isotype: IgG2b

Application Details

Application Notes: WB: 0.5 - 2 μg/mL dilution

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-DNMT3B antibody (ABIN2668953)

Felle, Joppien, Németh, Diermeier, Thalhammer, Dobner, Kremmer, Kappler, Längst: "The USP7/Dnmt1 complex stimulates the DNA methylation activity of Dnmt1 and regulates the stability of UHRF1." in: Nucleic acids research, Vol. 39, Issue 19, pp. 8355-65, (2011) (PubMed).

Target Details for DNMT3B

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Alternative Name: DNMT3B

Background: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 Beta) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2 and DNMT3. The DNMT3 family contains two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L). DNMT3B has been shown to be important in the regulation of specific patterns of DNA methylation, specifically de novo methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression. Mutations in the DNMT3B gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome.

Molecular Weight: 96 kDa

Gene ID: 1789

NCBI Accession: NP_787044