PIWIL2 antibody

Catalog No. ABIN2668966

There is 1 publication for this product available. The Mouse Monoclonal anti-PIWIL2 antibody is suitable to detect PIWIL2 in samples from Mouse. It has been validated for WB, ICC, IF and IP.

Quick Overview for PIWIL2 antibody (ABIN2668966)

Target: PIWIL2 (Piwi-Like 2 (PIWIL2))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PIWIL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 17-8

Product Details anti-PIWIL2 Antibody

Purpose: Mili / PiwiL2 antibody (mAb) (Clone 17.8)

Purification: Protein G Chromatography

Immunogen: This Mili / PiwiL2 antibody was raised against full-length recombinant mouse MILI / PIWIL2 protein.

Isotype: IgG1

Application Details

Application Notes: This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91253.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-PIWIL2 antibody (ABIN2668966)

Kirino, Kim, de Planell-Saguer, Khandros, Chiorean, Klein, Rigoutsos, Jongens, Mourelatos: "Arginine methylation of Piwi proteins catalysed by dPRMT5 is required for Ago3 and Aub stability." in: Nature cell biology, Vol. 11, Issue 5, pp. 652-8, (2009) (PubMed).

Target Details for PIWIL2

Target: PIWIL2 (Piwi-Like 2 (PIWIL2))

Alternative Name: Mili / PiwiL2

Background: Mili / PiwiL2 is a member of the PIWI/AGO subfamily of RNA-binding proteins. Mili / PiwiL2 contains a Piwi and an AGO domain and binds a class of RNA called piwi-interacting RNA (piRNA), which are 24 to 30 nucleotides in length and are derived primarily derived from transposons and other repeated sequence elements. Mili / PiwiL2 is required for methylation and inactivation of retrotransposons. Mice deficient in Mili / PiwiL2 are sterile, as it is also required for spermatogenesis. It also positively regulates translation, and is required for germ line stem cell division and differentiation.

Molecular Weight: 97 kDa

Gene ID: 57746

NCBI Accession: NP_001129193

Pathways: Stem Cell Maintenance