5-Methylcytosine (5MeC) antibody

Catalog No. ABIN2668983

This Mouse Monoclonal antibody specifically detects 5-Methylcytosine in IF, MeDIP and DB.

Quick Overview for 5-Methylcytosine (5MeC) antibody (ABIN2668983)

Target: 5-Methylcytosine (5MeC)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Methylated DNA Immunoprecipitation (MeDIP), Dot Blot (DB)

Clone: A1

Product Details

Purpose: 5-Methylcytosine (5-mC) antibody (mAb) (Clone A1)

Purification: Protein A Chromatography

Immunogen: This 5-Methylcytosine (5-mC) antibody was raised against 5-Methyl-cytidine conjugated to KLH and recognizes 5-Methylcytosine.

Isotype: IgG1

Application Details

Application Notes: MeDIP: 1 - 2 μg per IP MeDIP-Seq: 1 - 2 μg each DB: 0.5 - 2 μg/mL dilution In MeDIP, the DNA must be denatured when using this antibody. For 5-methylcytosine, we also offer AbFlex 5-methylcytosine Recombinant Antibody (rAb). For details, see Catalog No. 91187.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details

Target: 5-Methylcytosine (5MeC)

Alternative Name: 5-Methylcytosine

Target Type: Chemical

Background: 5-Methylcytosine (5-mC) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody (Clone A1) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.