Ubiquitin antibody

Catalog No. ABIN2668990

There is 1 publication for this product available. The Mouse Monoclonal anti-Ubiquitin antibody is suitable to detect Ubiquitin in samples from Human. It has been validated for WB.

Quick Overview for Ubiquitin antibody (ABIN2668990)

Target: Ubiquitin (Ub)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB)

Clone: P4D1

Product Details anti-Ubiquitin Antibody

Purpose: Ubiquitin antibody (mAb) (Clone P4D1)

Purification: Protein A Chromatography

Immunogen: This Ubiquitin antibody was raised against purified bovine ubiquitin.

Isotype: IgG

Application Details

Application Notes: WB For additional information, please see the original publication for the generation of this clone: Kahana, A., and Gottschling, D.E., Mol. Cell. Biol. 19: 6608-6620, 1999. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91257.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: PBS, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Ubiquitin antibody (ABIN2668990)

Kahana, Gottschling: "DOT4 links silencing and cell growth in Saccharomyces cerevisiae." in: Molecular and cellular biology, Vol. 19, Issue 10, pp. 6608-20, (2000) (PubMed).

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background: Ubiquitin is an important signaling protein that is catalytically added to proteins on lysine residues to modify their function. Addition of multiple ubiquitin proteins in a string (poly-ubiquitylation) to proteins signals their destruction by the proteasome. Mono-ubiquitylation of proteins has a much different effect, more in keeping with post-translational modification of proteins by phosphorylation or acetylation. Mono-ubiquitylation is part of a number of signal transduction pathways, including those involving histone proteins and transcriptional regulation.

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway