C3a antibody (Biotin)

Catalog No. ABIN2688861

There are 2+ publications for this product available. The Rat Monoclonal anti-C3a antibody is suitable to detect C3a in samples from Mouse. It has been validated for ELISA.

Quick Overview for C3a antibody (Biotin) (ABIN2688861)

Target: C3a (Complement Fragment 3a (C3a))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This C3a antibody is conjugated to Biotin

Application: ELISA

Clone: I87

Product Details anti-C3a Antibody (Biotin)

Characteristics: The I87-419 antibody reacts with the mouse C3a protein. Anaphylatoxin C3a is a bioactive cleavage product released from plasma component C3 during complement activation and is involved in mediation of a variety of cellular immune responses, as well as being a potent pro-inflammatory agent. The release of this cleavage product is a reliable indicator of in vivo or in vitro complement activation. This antibody is routinely tested by ELISA Detection. Other applications were tested by BD Biosciences Pharmingen during antibody development only or reported in the literature. Representative Mouse C3a standard curve. The curve was generated by a sandwich ELISA using the purified I87-1162 antibody as capture antibody, doubling dilutions of purified mouse C3a protein as standard, and biotinylated I87-419 antibody as detection antibody. The standard curve is displayed as the concentration of purified mouse C3a in ng/mL versus the microwell absorbance.

BD Pharmingen™ Biotin Rat Anti-Mouse C3a - Biotin - Clone I87-419 - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-C3a antibody (Biotin) (ABIN2688861)

Hugli: "Structure and function of C3a anaphylatoxin." in: Current topics in microbiology and immunology, Vol. 153, pp. 181-208, (1990) (PubMed).

Hugli: "Structure and function of the anaphylatoxins." in: Springer seminars in immunopathology, Vol. 7, Issue 2-3, pp. 193-219, (1984) (PubMed).

Target Details for C3a

Target: C3a (Complement Fragment 3a (C3a))

Alternative Name: C3a

Pathways: Complement System