Cardiac Troponin T2 antibody

Catalog No. ABIN2688865

This anti-Cardiac Troponin T2 antibody is a Mouse Monoclonal antibody detecting Cardiac Troponin T2 in WB, ICS and IHC (f). Suitable for Human, Mouse, Rat, Pig, Rabbit, Dog, Chicken and Guinea Pig. This Primary Antibody has been cited in 7+ publications.

Quick Overview for Cardiac Troponin T2 antibody (ABIN2688865)

Target: Cardiac Troponin T2 (cTnT) (Cardiac Troponin T (cTnT))

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Dog, Chicken, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cardiac Troponin T2 antibody is un-conjugated

Application: Western Blotting (WB), Intracellular Staining (ICS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 13

Product Details anti-Cardiac Troponin T2 Antibody

Brand: BD Pharmingen™

Characteristics: The 13-11 monoclonal antibody specifically recognizes Troponin T type 2 (cardiac), encoded by the gene TNNT2. Troponin T is the tropomyosin-binding subunit of the troponin complex, which also encompasses troponin C and troponin I. This complex regulates muscle contraction in skeletal and cardiac muscle in response to alterations in calcium levels. Troponin T type 2 is solely found in the heart, and genetic alterations in the TNNT2 gene are associated to a series of heart disorders in humans, including hypertrophic cardiomyopathy, dilated cardiomyopathy and left ventricular noncompaction. Cardiac Troponin T can be used as a marker for the identification of cardiomyocytes derived from pluripotent stem cells. LEFT PANEL: Western blot analysis of Cardiac Troponin T expression in human and mouse tissues. Human heart lysate was obtained from Abcam (Cat .No. ab2943, lane 1). C2C12 mouse myoblasts (ATCC CRL-1772) were cultured in DMEM containing 10 % FBS (lane 2) or 0.5 % FBS for 14 days for induction of cell differentiation (lane 3). Lysates were prepared, electrophoresed (SDS-PAGE) and transferred to PVDF membranes. The membranes were labeled with 1 μg/mL of Purified Mouse Anti-Cardiac Troponin T. Specific staining was detected with HRP Goat Anti-Mouse Ig (Cat. No. 554002). RIGHT PANEL: Immunohistochemical staining of Cardiac Troponin T in human heart. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No.550878, left) or Purified Mouse Anti-Cardiac Troponin T (right). A three-step staining procedure that employs Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin HRP (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification: 40x. Flow cytometric analysis of Cardiac Troponin T in human (left panel) and mouse (right panel) cardiomyocytes differentiated in vitro. Human embryonic stem cell-derived cardiomyocytes (Evans lab, UCSD) were disassociated and fixed in BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm/Wash buffer I (Cat. No. 557885). The C2C12 mouse myoblast cell line (ATCC CRL-1772) was cultured for 5 days in low-serum conditions for induction of cell differentiation, fixed with BD Cytofix™ Fixation Buffer, and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121, dashed line) or Purified Mouse Anti-Cardiac Troponin T (solid line). The second-step reagents were FITC Goat Anti-Mouse Ig (Cat. No. 554001, left) and PE Goat Anti-Mouse Ig (Cat. No. 550589, right). Events with the forward and side light-scatter characteristics of intact cells were gated. Flow cytometry was performed on a BD LSR Fortessa™ (left) or a BD FACSCanto™ II (right) flow cytometry system.

BD Pharmingen™ Purified Mouse Anti-Cardiac Troponin T - Purified - Clone 13-11 - Isotype Mouse IgG1, κ - Reactivity Ms, Hu, Chick, Dog, G Pig, Pig, Rab, Rat - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rabbit cardiac troponin T Protein

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Cardiac Troponin T2 antibody (ABIN2688865)

Jáchymová, Muravská, Paleček, Kuchynka, Řeháková, Magage, Kr��l, Zima, Horký, Linhart: "Genetic variation screening of TNNT2 gene in a cohort of patients with hypertrophic and dilated cardiomyopathy." in: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 61, Issue 2, pp. 169-75, (2012) (PubMed).

Lian, Hsiao, Wilson, Zhu, Hazeltine, Azarin, Raval, Zhang, Kamp, Palecek: "Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 27, pp. E1848-57, (2012) (PubMed).

Uosaki, Fukushima, Takeuchi, Matsuoka, Nakatsuji, Yamanaka, Yamashita: "Efficient and scalable purification of cardiomyocytes from human embryonic and induced pluripotent stem cells by VCAM1 surface expression." in: PLoS ONE, Vol. 6, Issue 8, pp. e23657, (2011) (PubMed).

Zhang, Wilson, Soerens, Koonce, Yu, Palecek, Thomson, Kamp: "Functional cardiomyocytes derived from human induced pluripotent stem cells." in: Circulation research, Vol. 104, Issue 4, pp. e30-41, (2009) (PubMed).

Mauritz, Schwanke, Reppel, Neef, Katsirntaki, Maier, Nguemo, Menke, Haustein, Hescheler, Hasenfuss, Martin: "Generation of functional murine cardiac myocytes from induced pluripotent stem cells." in: Circulation, Vol. 118, Issue 5, pp. 507-17, (2008) (PubMed).

Thierfelder, Watkins, MacRae, Lamas, McKenna, Vosberg, Seidman, Seidman: "Alpha-tropomyosin and cardiac troponin T mutations cause familial hypertrophic cardiomyopathy: a disease of the sarcomere." in: Cell, Vol. 77, Issue 5, pp. 701-12, (1994) (PubMed).

Malouf, McMahon, Oakeley, Anderson: "A cardiac troponin T epitope conserved across phyla." in: The Journal of biological chemistry, Vol. 267, Issue 13, pp. 9269-74, (1992) (PubMed).

Target Details for Cardiac Troponin T2

Target: Cardiac Troponin T2 (cTnT) (Cardiac Troponin T (cTnT))

Alternative Name: Cardiac Troponin T

Background: Synonyms: cTnT, Cardiac Muscle Troponin T, Troponin T type 2, TNNT2

Molecular Weight: 35-40 kDa