ICAM2 antibody

Catalog No. ABIN2688871

Cited in 3+ publications. The Rat Monoclonal anti-ICAM2 antibody (Clone 3C4) (ABIN2688871) specifically detects ICAM2 in FACS, IP, IHC (fro), IHC (p) and BR. The antibody is reactive with Mouse samples.

Quick Overview for ICAM2 antibody (ABIN2688871)

Target: ICAM2 (Intercellular Adhesion Molecule 2 (ICAM2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ICAM2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Blocking Reagent (BR)

Clone: 3C4

Product Details anti-ICAM2 Antibody

Characteristics: The 3C4 (mIC2/4) antibody reacts with the mouse ICAM-2 (CD102) cell surface glycoprotein, a ligand for LFA-1. CD102 is constitutively expressed on endothelial cells, T and B lymphocytes, and alveolar walls. It is also expressed on a variety of leukocyte cell lines. CD102 does not appear to be involved in the development of hematopoietic cells. In a model for allergic asthma, endothelial CD102 mediates the transmigration of eosinophils (but not lymphocytes, monocytes, or macrophages) into the airway lumen. The 3C4 (mIC2/4) antibody blocks interactions between ICAM-2 and LFA-1. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse CD102 - Purified - Clone 3C4(mIC2/4) - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Transfected Cell Line

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-ICAM2 antibody (ABIN2688871)

Gerwin, Gonzalo, Lloyd, Coyle, Reiss, Banu, Wang, Xu, Avraham, Engelhardt, Springer, Gutierrez-Ramos: "Prolonged eosinophil accumulation in allergic lung interstitium of ICAM-2 deficient mice results in extended hyperresponsiveness." in: Immunity, Vol. 10, Issue 1, pp. 9-19, (1999) (PubMed).

Xu, Bickford, Luther, Carpenito, Takei, Springer: "Characterization of murine intercellular adhesion molecule-2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 12, pp. 4909-14, (1996) (PubMed).

Xu, Tong, De Fougerolles, Springer: "Isolation, characterization, and expression of mouse ICAM-2 complementary and genomic DNA." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 8, pp. 2650-5, (1992) (PubMed).

Target Details for ICAM2

Target: ICAM2 (Intercellular Adhesion Molecule 2 (ICAM2))

Alternative Name: CD102

Background: Synonyms: ICAM-2