ITGAL antibody

Catalog No. ABIN2688903

Product Details anti-ITGAL Antibody

Target: ITGAL (Integrin alpha-L (ITGAL))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGAL antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Brand: BD Pharmingen™

Characteristics: The WT.5 antibody reacts with the asubunit of LFA-1 (αLβ2 integrin, CD11a/CD18), a heterodimeric surface glycoprotein which is found on the majority of leukocytes, but not on peritoneal macrophages or peritoneal mast cells. LFA-1 mediates a variety of heterotypic and homotypic intercellular adhesions through interaction with ICAM-1 (CD54) and ICAM-2 (CD102). WT.1 mAb recognizes both the activated and unactivated forms of LFA-1. It inhibits the binding of LFA-1 to ICAM-1 in several in vitro assays, including binding of Concanavalin A-stimulated lymphocytes (Con A blasts) to purified ICAM-1 and Mg2+-dependent aggregation of concanavalin A-stimulated blasts. It has also been reported to inhibit leukocyte infiltration in several in vivo models of inflammation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Mouse Anti-Rat CD11a - Purified - Clone WT.1 - Isotype Mouse IgG2a, κ - Reactivity Rat - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: PHA-stimulated rat splenocytes and rat thymic lymphoma FTL-43

Clone: WT-1

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-ITGAL antibody (ABIN2688903)

Ohta, Gotoh, Ohzato, Fukuzaki, Nishihara, Dono, Umeshita, Sakon, Yagita, Okumura, Tanaka, Kawashima, Miyasaka, Monden: "Direct antigen presentation through binding of donor intercellular adhesion molecule-1 to recipient lymphocyte function-associated antigen-1 molecules in xenograft rejection." in: Transplantation, Vol. 65, Issue 8, pp. 1094-100, (1998) (PubMed).

Andrews, Malcontenti-Wilson, OBrien: "Expression of adhesion molecules and leukocyte recruitment into gastric mucosa following ischemia-reperfusion." in: Digestive diseases and sciences, Vol. 42, Issue 2, pp. 326-32, (1997) (PubMed).

Watanabe, Arakawa, Fukuda, Higuchi, Kobayashi: "Role of neutrophils in a rat model of gastric ulcer recurrence caused by interleukin-1 beta." in: The American journal of pathology, Vol. 150, Issue 3, pp. 971-9, (1997) (PubMed).

Wada, Shikata, Makino, Morioka, Hirata, Ota, Tamatani, Miyasaka, Horiuchi, Noji, Nishikawa, Myokai, Taniguchi, Kanwar, Ota: "The critical role of intercellular adhesion molecule-1 in Masugi nephritis in rats." in: Nephron, Vol. 73, Issue 2, pp. 264-72, (1996) (PubMed).

Devine, Lightman, Greenwood: "Role of LFA-1, ICAM-1, VLA-4 and VCAM-1 in lymphocyte migration across retinal pigment epithelial monolayers in vitro." in: Immunology, Vol. 88, Issue 3, pp. 456-62, (1996) (PubMed).

Fox, Jewell, Whitacre: "Rat peritoneal mast cells present antigen to a PPD-specific T cell line." in: Cellular immunology, Vol. 158, Issue 1, pp. 253-64, (1994) (PubMed).

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, (1994) (PubMed).

Bañuls, Alvarez, Ferrero, Zapata, Ardavin: "Cell-surface marker analysis of rat thymic dendritic cells." in: Immunology, Vol. 79, Issue 2, pp. 298-304, (1993) (PubMed).

Yamazaki, Seko, Tamatani, Miyasaka, Yagita, Okumura, Nagai, Yazaki: "Expression of intercellular adhesion molecule-1 in rat heart with ischemia/reperfusion and limitation of infarct size by treatment with antibodies against cell adhesion molecules." in: The American journal of pathology, Vol. 143, Issue 2, pp. 410-8, (1993) (PubMed).

Tamatani, Kotani, Miyasaka: "Characterization of the rat leukocyte integrin, CD11/CD18, by the use of LFA-1 subunit-specific monoclonal antibodies." in: European journal of immunology, Vol. 21, Issue 3, pp. 627-33, (1991) (PubMed).

Larson, Springer: "Structure and function of leukocyte integrins." in: Immunological reviews, Vol. 114, pp. 181-217, (1990) (PubMed).

Target Details for ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Alternative Name: CD11a (ITGAL Products)

Synonyms: CD11A antibody, LFA-1 antibody, LFA1A antibody, (p180) antibody, Cd11a antibody, LFA-1A antibody, Ly-15 antibody, Ly-21 antibody, CD11a antibody, ITGAL antibody, integrin subunit alpha L antibody, integrin alpha L antibody, ITGAL antibody, Itgal antibody

Background: Synonyms: Integrin αL chain, LFA-1 α chain

Pathways: Activated T Cell Proliferation, Integrin Complex