IL3RA antibody (Biotin)

Catalog No. ABIN2688936

The Mouse Monoclonal anti-IL3RA antibody has been validated for FACS and FM. It is suitable to detect IL3RA in samples from Human. There are 3+ publications available.

Quick Overview for IL3RA antibody (Biotin) (ABIN2688936)

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL3RA antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Clone: 9F5

Product Details anti-IL3RA Antibody (Biotin)

Brand: BD Pharmingen™

Characteristics: Reacts with the 70 kDa IL-3 receptor α chain(IL-3Rα), which associates with the 120-140 kDa β subunit. The β chain is shared with the receptors for interleukins IL-5 and GM-CSF. IL-3Rα is expressed on myeloid precursors, basophils, mast cells, macrophages, and megakaryocytes. Reports indicate that IL-3Rα is also expressed on lymphocytes. IL-3R plays an important role in hematopoietic progenitor cell growth and differentiation. This antibody does not block binding of IL-3 to the IL-3 receptor. Profile of TF-1 (erythroid leukemia) cells analyzed on a FACScan

BD Pharmingen™ Biotin Mouse Anti-Human CD123 - Biotin - Clone 9F5 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-IL3RA antibody (Biotin) (ABIN2688936)

Macardle, Chen, Shih, Huang, Weedon, Sun, Lopez, Zola: "Characterization of human leucocytes bearing the IL-3 receptor." in: Cellular immunology, Vol. 168, Issue 1, pp. 59-68, (1996) (PubMed).

Sun, Woodcock, Rapoport, Stomski, Korpelainen, Bagley, Goodall, Smith, Gamble, Vadas, Lopez: "Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist." in: Blood, Vol. 87, Issue 1, pp. 83-92, (1996) (PubMed).

Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

Target Details for IL3RA

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Alternative Name: CD123

Background: Synonyms: IL-3 Receptor α chain

Pathways: JAK-STAT Signaling