Syndecan 1 antibody (Biotin)

Catalog No. ABIN2688968

This Rat Monoclonal antibody specifically detects Syndecan 1 in FACS, IF and IHC (fro). It exhibits reactivity toward Mouseand has been mentioned in 1 publication.

Quick Overview for Syndecan 1 antibody (Biotin) (ABIN2688968)

Target: Syndecan 1 (SDC1)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Syndecan 1 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 281

Product Details anti-Syndecan 1 Antibody (Biotin)

Brand: BD Pharmingen™

Characteristics: The 281-2 antibody reacts with the core protein of CD138 (Syndecan-1), a cell-surface, integral membrane heparan sulfate- and chondroitin sulfate-containing proteoglycan that binds to interstitial extracellular matrix molecules. Syndecan-1 is predominantly expressed on epithelial cells, where its expression correlates with normal epithelial organization. It is also expressed on B lymphocytes at specific stages during their differentiation: precursor B cells in the bone marrow and antibody-secreting cells, including plasma cells, but not mature peripheral B cells. It is thus implicated in mediating B cell-matrix interactions. CD138 expression is also regulated during embryonic development, and the molecule shows a tissue- specific structural polymorphism resulting from different post-translational modifications. The 281-2 antibody may be used to detect the differently glycosylated forms, because it reacts with the core protein. Furthermore, the mAb detects the Syndecan-1 ectodomain which is cleaved from cell surfaces by a metalloproteinase. Expression of CD138 on mouse bone- arrow B lymphocytes. C57BL/6 bone- arrow leukocytes were simultaneously stained with biotin-conjugated 281-2 mAb (Right panel) and FITC-conjugated RA3-6B2 mAb (anti- D45R/B220, Cat. no. 553087/553088, both panels), followed by Streptavidin-PE (Cat. No. 554061, both panels). Flow cytometry was performed on a BD FACScan™

BD Pharmingen™ Biotin Mouse Anti-CD138 - Biotin - Clone 281-2 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: NAMRU mouse mammary gland epithelial cell line NMuMG

Isotype: IgG2a kappa

Application Details

Application Notes: Flow Cytometry System.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-Syndecan 1 antibody (Biotin) (ABIN2688968)

Wehrli, Legler, Finke, Toellner, Loetscher, Baggiolini, MacLennan, Acha-Orbea: "Changing responsiveness to chemokines allows medullary plasmablasts to leave lymph nodes." in: European journal of immunology, Vol. 31, Issue 2, pp. 609-16, (2001) (PubMed).

Target Details for Syndecan 1

Target: Syndecan 1 (SDC1)

Alternative Name: CD138

Background: Synonyms: Syndecan-1

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism