CD16 antibody (Biotin)

Catalog No. ABIN2689005

There are 4+ publications for this product available. The Mouse Monoclonal anti-CD16 antibody is suitable to detect CD16 in samples from Pig. It has been validated for FACS.

Quick Overview for CD16 antibody (Biotin) (ABIN2689005)

Target: CD16

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: FcG7

Product Details anti-CD16 Antibody (Biotin)

Characteristics: The FcG7 antibody reacts with CD16, the transmembrane α chain of Fcγ Receptor III (FcγRIIIAα). In the pig, FcγRIIIAα is expressed on NK cells, monocytes, granulocytes, and pulmonary alveolar macrophages. The FcG7 antibody induces redirected cytotoxic activity of NK cells and granulocytes against tumor cells. Two-color analysis of CD16 expression on pig peripheral blood lymphocytes. Pig whole blood was stained with PE-conjugated anti- pig CD4a mAb 74-12-4 (Cat. no. 559586, both panels) and biotinylated mAb FcG7 (Right panel), followed by Streptavidin- FITC (Cat. No. 554060, both panels). Erythrocytes were lysed (PharmLyse™ Lysing Buffer, Cat. no. 555899), non-viable leukocytes were excluded by staining with 7-AAD (Via- Probe™, Cat. No. 555816/555815), and lymphocytes were gated according to their light-scatter profiles. Flow cytometry was performed on a FACSCalibura .

BD Pharmingen™ Biotin Mouse Anti-Pig CD16 - Biotin - Clone FcG7 - Isotype Mouse IgG1, κ - Reactivity Pig - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Pig peripheral blood leukocytes

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD16 antibody (Biotin) (ABIN2689005)

Aller, Cho, Kim: "Characterization of the cytolytic trigger molecules G7/PNK-E as a molecular complex on the surface of porcine phagocytes." in: Cellular immunology, Vol. 161, Issue 2, pp. 270-8, (1995) (PubMed).

Halloran, Sweeney, Strohmeier, Kim: "Molecular cloning and identification of the porcine cytolytic trigger molecule G7 as a Fc gamma RIII alpha (CD16) homologue." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 6, pp. 2631-41, (1994) (PubMed).

Halloran, Sweeney, Kim: "Biochemical characterization of the porcine Fc gamma RIII alpha homologue G7." in: Cellular immunology, Vol. 158, Issue 2, pp. 400-13, (1994) (PubMed).

Dato, Wierda, Kim: "A triggering structure recognized by G7 monoclonal antibody on porcine lymphocytes and granulocytes." in: Cellular immunology, Vol. 140, Issue 2, pp. 468-77, (1992) (PubMed).

Target Details for CD16

Target: CD16

Alternative Name: CD16

Background: Synonyms: Fcγ Receptor IIIAα

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process