L1CAM antibody

Catalog No. ABIN2689016

Product Details anti-L1CAM Antibody

Target: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This L1CAM antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: Neurite adhesion molecule L1 has been implicated in neuron-neuron and neuron-Schwann cell adhesion in vertebrates. L1-like molecules, found in mouse, rat, chicken, and human, promote axonal elongation and may also play a role in regeneration of axons after injury. Molecular cloning data suggest 87 % amino acid identity between mouse and human L1 Molecules. 5G3 antigen (Ag), originally defined by monoclonal antibody 5G3, is considered to be the human homologue of mouse L1. The 5G3 antibody was developed against a human neuroblastoma cell line to use as a probe for the elucidating the biological characteristics of neuroblastoma. 5G3 specifically recognizes a neuroblastoma target glycoprotein antigen of 215 kDa and its 200 kDa precursor. The 215 kDa molecule is expressed on the cell surface, whereas the 200 kDa precursor is shed from the cell surface. The 215 and 200 kDa species also differ in their posttranslational modification patterns. The 5G3 antibody has been used as a marker for neuroblastoma, and to purify 5G3 Ag from normal adult human brain. The antibody recognizes human L1 on human neuroblastoma cell lines and tissues. Reactivity has been tested on a variety of malignant and normal tissues. Squamous lung, squamous skin, and osteogenic sarcoma cell lines were positive, as were two out of eight melanoma cell lines tested. A variety of other cell lines and tumor tissues tested negative. 5G3 did not react with either T or B lymphoblastoid cell lines or a fibroblast cell line. Among all the normal tissues tested, mAb 5G3 reacted only with cerebellum. The molecular masses observed using mAb 5G3 may vary among immunoprecipitation isolates. In normal human cerebellum, 5G3 Ag migrated as a 190/200 kDa doublet, 140 kDa band with minor bands at 80 and 65 kDa. 5G3 Ag isolated from SK-N-AS cells migrates as 200 to 215 kDa bands, or as a diffuse band ranging from 200 to 215 kDa. Additional bands have been described at 140 to 150 kDa in SK-N-AS cells. Only the 200 kDa band has been detected in culture media from SK-N-AS cells. Profile of M21 human melanoma cells analyzed by flow cytometry using clone 5G3. Cells were incubated with either clone 5G3 (Cat. No. 554273) or with an isotype control, and then a FITC-conjugated second step antibody.

BD Pharmingen™ Purified Mouse Anti-Human CD171 - Purified - Clone 5G3 - Isotype Mouse IgG2a - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Neuroblastoma SK-N-AS

Clone: 5G3

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-L1CAM antibody (ABIN2689016)

Mechtersheimer, Gutwein, Agmon-Levin, Stoeck, Oleszewski, Riedle, Postina, Fahrenholz, Fogel, Lemmon, Altevogt: "Ectodomain shedding of L1 adhesion molecule promotes cell migration by autocrine binding to integrins." in: The Journal of cell biology, Vol. 155, Issue 4, pp. 661-73, (2001) (PubMed).

Nayeem, Silletti, Yang, Lemmon, Reisfeld, Stallcup, Montgomery: "A potential role for the plasmin(ogen) system in the posttranslational cleavage of the neural cell adhesion molecule L1." in: Journal of cell science, Vol. 112 ( Pt 24), pp. 4739-49, (2000) (PubMed).

Reid, Hemperly: "Variants of human L1 cell adhesion molecule arise through alternate splicing of RNA." in: Journal of molecular neuroscience : MN, Vol. 3, Issue 3, pp. 127-35, (1992) (PubMed).

Rathjen, Wolff, Chang, Bonhoeffer, Raper: "Neurofascin: a novel chick cell-surface glycoprotein involved in neurite-neurite interactions." in: Cell, Vol. 51, Issue 5, pp. 841-9, (1988) (PubMed).

Lemmon, McLoon: "The appearance of an L1-like molecule in the chick primary visual pathway." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 6, Issue 10, pp. 2987-94, (1986) (PubMed).

Mujoo, Spiro, Reisfeld: "Characterization of a unique glycoprotein antigen expressed on the surface of human neuroblastoma cells." in: The Journal of biological chemistry, Vol. 261, Issue 22, pp. 10299-305, (1986) (PubMed).

Wolff, Plow, Ginsberg: "Interaction of thrombospondin with resting and stimulated human platelets." in: The Journal of biological chemistry, Vol. 261, Issue 15, pp. 6840-6, (1986) (PubMed).

Cheresh, Honsik, Staffileno, Jung, Reisfeld: "Disialoganglioside GD3 on human melanoma serves as a relevant target antigen for monoclonal antibody-mediated tumor cytolysis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 15, pp. 5155-9, (1985) (PubMed).

Rathjen, Schachner: "Immunocytological and biochemical characterization of a new neuronal cell surface component (L1 antigen) which is involved in cell adhesion." in: The EMBO journal, Vol. 3, Issue 1, pp. 1-10, (1984) (PubMed).

Salton, Shelanski, Greene: "Biochemical properties of the nerve growth factor-inducible large external (NILE) glycoprotein." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 3, Issue 12, pp. 2420-30, (1984) (PubMed).

Target Details for L1CAM

Target: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Alternative Name: CD171 (L1CAM Products)

Synonyms: l1cam-a antibody, CAML1 antibody, CD171 antibody, HSAS antibody, HSAS1 antibody, MASA antibody, MIC5 antibody, N-CAM-L1 antibody, N-CAML1 antibody, NCAM-L1 antibody, S10 antibody, SPG1 antibody, L1 antibody, Hsas antibody, Hyd antibody, NCAML1 antibody, L1 cell adhesion molecule S homeolog antibody, L1 cell adhesion molecule antibody, l1cam.S antibody, L1CAM antibody, L1cam antibody

Background: Synonyms: L1 Neuite Cell Adhesion Molecule

Pathways: Synaptic Membrane