CCR4 antibody (Chemokine (C-C Motif) Receptor 4)

Details for Product anti-CCR4 Antibody No. ABIN2689052, Supplier: Log in to see
Antigen
  • CC-CKR-4
  • CD194
  • CKR4
  • CMKBR4
  • ChemR13
  • HGCN:14099
  • K5-5
  • Ccr4
  • C-C CKR-4
  • CHEMR1
  • Cmkbr4
  • LESTR
  • Sdf1r
  • C-C motif chemokine receptor 4
  • chemokine (C-C motif) receptor 4
  • CCR4
  • Ccr4
Reactivity
Human
198
66
46
9
8
6
3
2
2
Host
Mouse
126
71
18
9
Clonality (Clone)
Monoclonal ()
Conjugate
This CCR4 antibody is un-conjugated
10
8
8
8
7
4
4
4
4
4
4
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
94
88
72
43
40
15
14
10
8
6
6
5
5
4
3
3
3
2
1
Options
Supplier
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Supplier Product No.
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Brand BD Pharmingen™
Immunogen Human CCR4 Transfected Cell Line
Clone 1G1
Isotype IgG1, kappa
Characteristics The monoclonal antibody 1G1 reacts with CD194, also known as the human CC Chemokine Receptor type 4 (CCR4). CCR4 is expressed on activated Th2 cells, regulatory T cells, activated NK cells, basophils, monocytes and platelets. CCR4 is a seven-transmembrane, G-protein-coupled receptor, and is the specific receptor for CC chemokines, CCL22/MDC/Macrophage-Derived Chemokine and CCL17/TARC/Thymus and Activation-Regulated Chemokine. It has been reported that CCR4 mRNA is expressed mainly in the thymus and spleen. The human CCR4 gene has been mapped to chromosome 3p24. The purified form of this antibody has been reported not to be a neutralizing antibody. The immunogen used to generate the 1G1 hybridoma has been reported to be human CCR4 transfected L1.2 mouse lymphoma cells. Flow cytometric detection of CD194 (CCR4) on human peripheral lymphocytes. Human PBMC were stained with 0.25 μg of the purified Mouse Anti-Human CD194 (CCR4) antibody using a Biotin Goat Anti-Mouse Ig secondary antibody and with PE Streptavidin in conjunction with either a FITC Mouse Anti-Human CD4 antibody (Cat. No. 555346, left panel) or with a FITC Mouse Anti-Human CD8 antibody (Cat. No. 555366, right panel). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using purified mouse IgG1 (Cat. No. 555746) as an isotype control. The quadrant markers for the bivariate dot plots were set based on the isotype control.

BD Pharmingen™ Purified Mouse Anti-Human CD194 - Purified - Clone 1G1 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Plasmids, Primers & others Plasmids, Primers & others CCR4 products on genomics-online (e.g. as negative or positive controls)
Alternative Name CD194 (CCR4 Antibody Abstract)
Background Synonyms: CCR4, C-C chemokine receptor type 4, CMKBR4, K5-5
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Product cited in: Campbell, Haraldsen, Pan, Rottman, Qin, Ponath, Andrew, Warnke, Ruffing, Kassam, Wu, Butcher: "The chemokine receptor CCR4 in vascular recognition by cutaneous but not intestinal memory T cells." in: Nature, Vol. 400, Issue 6746, pp. 776-80, 1999 (PubMed).

DAmbrosio, Iellem, Bonecchi, Mazzeo, Sozzani, Mantovani, Sinigaglia: "Selective up-regulation of chemokine receptors CCR4 and CCR8 upon activation of polarized human type 2 Th cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 10, pp. 5111-5, 1998 (PubMed).

Sallusto, Lenig, Mackay, Lanzavecchia: "Flexible programs of chemokine receptor expression on human polarized T helper 1 and 2 lymphocytes." in: The Journal of experimental medicine, Vol. 187, Issue 6, pp. 875-83, 1998 (PubMed).

Imai, Chantry, Raport, Wood, Nishimura, Godiska, Yoshie, Gray: "Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4." in: The Journal of biological chemistry, Vol. 273, Issue 3, pp. 1764-8, 1998 (PubMed).

Samson, Soularue, Vassart, Parmentier: "The genes encoding the human CC-chemokine receptors CC-CKR1 to CC-CKR5 (CMKBR1-CMKBR5) are clustered in the p21.3-p24 region of chromosome 3." in: Genomics, Vol. 36, Issue 3, pp. 522-6, 1997 (PubMed).

Imai, Baba, Nishimura, Kakizaki, Takagi, Yoshie: "The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4." in: The Journal of biological chemistry, Vol. 272, Issue 23, pp. 15036-42, 1997 (PubMed).

Power, Meyer, Nemeth, Bacon, Hoogewerf, Proudfoot, Wells: "Molecular cloning and functional expression of a novel CC chemokine receptor cDNA from a human basophilic cell line." in: The Journal of biological chemistry, Vol. 270, Issue 33, pp. 19495-500, 1995 (PubMed).

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