CCR5 antibody

Catalog No. ABIN2689053

Product Details anti-CCR5 Antibody

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCR5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Brand: BD Pharmingen™

Characteristics: Reacts with the chemokine receptor, CCR5, a seven transmembrane-spanning G protein-associated molecule. 3A9 antibody has also been reported to cross-react with human CCR8. Results of epitope mapping and sequence comparison between CCR5 and CCR8 reveals that the first three amino acid residues for these two receptors are identical: MDY (Met-Asp-Tyr). CCR5 belongs to the β-chemokine receptor family. It is expressed on a subset of T lymphocytes (CD3+, CD45RO+, CD95+). CCR5 regulates lymphocyte chemotaxis activation and transendothelial migration during inflammation. It signals a response to at least three chemokines: RANTES and macrophage inflammatory protein-1 (MIP-1) α and β. Additionally, CCR5 has been found to be a co-receptor for macrophage-tropic HIV-1 on CD4+ cells, a characteristic that is important in viral transmission. Reports indicate that individuals who have partial (heterozygous) or complete (homozygous) deletion of the CCR5 allele, demonstrate resistance to HIV infection. CCR5 has been clustered as CD195 in the VIIth HLDA workshop. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed by flow cytometry

BD Pharmingen™ Purified Mouse Anti-Human CD195 - Purified - Clone 3A9 - Isotype Mouse IgG2a, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: 3A9

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CCR5 antibody (ABIN2689053)

Hancock: "Chemokines and the pathogenesis of T cell-dependent immune responses." in: The American journal of pathology, Vol. 148, Issue 3, pp. 681-4, (1997) (PubMed).

Rottman, Ganley, Williams, Wu, Mackay, Ringler: "Cellular localization of the chemokine receptor CCR5. Correlation to cellular targets of HIV-1 infection." in: The American journal of pathology, Vol. 151, Issue 5, pp. 1341-51, (1997) (PubMed).

Wu, Paxton, Kassam, Ruffing, Rottman, Sullivan, Choe, Sodroski, Newman, Koup, Mackay: "CCR5 levels and expression pattern correlate with infectability by macrophage-tropic HIV-1, in vitro." in: The Journal of experimental medicine, Vol. 185, Issue 9, pp. 1681-91, (1997) (PubMed).

Choe, Farzan, Sun, Sullivan, Rollins, Ponath, Wu, Mackay, LaRosa, Newman, Gerard, Gerard, Sodroski: "The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates." in: Cell, Vol. 85, Issue 7, pp. 1135-48, (1996) (PubMed).

Deng, Liu, Ellmeier, Choe, Unutmaz, Burkhart, Di Marzio, Marmon, Sutton, Hill, Davis, Peiper, Schall, Littman, Landau: "Identification of a major co-receptor for primary isolates of HIV-1." in: Nature, Vol. 381, Issue 6584, pp. 661-6, (1996) (PubMed).

Doranz, Rucker, Yi, Smyth, Samson, Peiper, Parmentier, Collman, Doms: "A dual-tropic primary HIV-1 isolate that uses fusin and the beta-chemokine receptors CKR-5, CKR-3, and CKR-2b as fusion cofactors." in: Cell, Vol. 85, Issue 7, pp. 1149-58, (1996) (PubMed).

Dragic, Litwin, Allaway, Martin, Huang, Nagashima, Cayanan, Maddon, Koup, Moore, Paxton: "HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5." in: Nature, Vol. 381, Issue 6584, pp. 667-73, (1996) (PubMed).

Raport, Gosling, Schweickart, Gray, Charo: "Molecular cloning and functional characterization of a novel human CC chemokine receptor (CCR5) for RANTES, MIP-1beta, and MIP-1alpha." in: The Journal of biological chemistry, Vol. 271, Issue 29, pp. 17161-6, (1996) (PubMed).

Target Details for CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Alternative Name: CD195 (CCR5 Products)

Synonyms: CC-CKR-5 antibody, CCCKR5 antibody, CCR-5 antibody, CD195 antibody, CKR-5 antibody, CKR5 antibody, CMKBR5 antibody, IDDM22 antibody, Ckr5 antibody, Cmkbr5 antibody, CCR1-L antibody, GPCR antibody, CCR5 antibody, AM4-7 antibody, C-C motif chemokine receptor 5 (gene/pseudogene) antibody, chemokine (C-C motif) receptor 5 antibody, C-C motif chemokine receptor 5 antibody, CCR5 antibody, Ccr5 antibody

Background: Synonyms: CCR5

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size