CD22.2 antibody (Biotin)
Reactivity: Mouse
IHC (fro), FACS, IP
Host: Mouse
Monoclonal
Cy34-1
Biotin
(9 references)-
- Target
- CD22.2
- Reactivity
- Mouse
- Host
- Mouse
- Clonality
- Monoclonal
- Conjugate
- Biotin
- Application
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
- Brand
- BD Pharmingen™
- Characteristics
-
The Cy34.1 antibody reacts with the B-lymphocyte differentiation antigen CD22 on strains having the Lyb-8.2 alloantigen (e.g., A, BALB/c, CBA, C3H/He, C57BL, C57L, C58, SJL, SWR, but not AKR, DBA/1, DBA/2, NZB, PL). CD22 is expressed at high levels on mature peripheral B lymphocytes (follicular and marginal zone), B-1 cells (CD5+ B cells), and plasma cells. It is a member of the Ig gene superfamily and associates with the B-cell antigen receptor. Its sialic acid- binding immunoglobulin-like lectin (siglec) extracellular region mediates B-cell adhesion to ligands on endothelial cells in the bone marrow. Its intracellular domain is phosphorylated after cross-linking of antigen receptor or MHC class II antigen. It is involved in negative regulation of B-cell activation and protection from autoimmunity. B-cell proliferative responses to LPS or anti-mouse Ig μ chain are augmented in the presence of Cy34.1 mAb. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in literature.
BD Pharmingen™ Biotin Mouse Anti-Mouse CD22.2 - Biotin - Clone Cy34.1 - Isotype Mouse IgG1, κ - Reactivity Ms - 0.5 mg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- B10.D2 mouse splenocytes
- Clone
- Cy34-1
- Isotype
- IgG1 kappa
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Regulation of MHC class II signal transduction by the B cell coreceptors CD19 and CD22." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 10, pp. 5588-96, (2000) (PubMed).
: "Dysregulated expression of the Cd22 gene as a result of a short interspersed nucleotide element insertion in Cd22a lupus-prone mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 6, pp. 2987-96, (2000) (PubMed).
: "Identification of CD22 ligands on bone marrow sinusoidal endothelium implicated in CD22-dependent homing of recirculating B cells." in: The Journal of experimental medicine, Vol. 189, Issue 9, pp. 1513-8, (1999) (PubMed).
: "Differential expression of CD22 (Lyb8) on murine B cells." in: International immunology, Vol. 8, Issue 7, pp. 1121-9, (1997) (PubMed).
: "Hyperresponsive B cells in CD22-deficient mice." in: Science (New York, N.Y.), Vol. 274, Issue 5288, pp. 798-801, (1996) (PubMed).
: "A role in B cell activation for CD22 and the protein tyrosine phosphatase SHP." in: Science (New York, N.Y.), Vol. 269, Issue 5221, pp. 242-4, (1995) (PubMed).
: "Regulation of lymphocyte activation by the cell-surface molecule CD22." in: Immunology today, Vol. 15, Issue 9, pp. 442-9, (1994) (PubMed).
: "Organization of the murine Cd22 locus. Mapping to chromosome 7 and characterization of two alleles." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 1, pp. 175-87, (1993) (PubMed).
: "Lyb-8.2: A new B cell antigen defined and characterized with a monoclonal antibody." in: Immunogenetics, Vol. 16, Issue 5, pp. 381-91, (1983) (PubMed).
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: "Regulation of MHC class II signal transduction by the B cell coreceptors CD19 and CD22." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 10, pp. 5588-96, (2000) (PubMed).
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- Target
- CD22.2
- Background
- Synonyms: Lyb-8.2
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