2B4 antibody

Catalog No. ABIN2689111

Product Details anti-2B4 Antibody

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This 2B4 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: Reacts with a 66 kDa single chain transmembrane glycoprotein. 2B4 belong to the immunoglobulin superfamily of proteins and is structurally related to CD2-like molecules. It was originally identified in the mouse as a non-MHC-restricted cytotoxicity mediator present on NK cells and CD8+ T cells. In humans, 2B4 is expressed on NK cells, CD8+ T cells, monocytes and basophils, suggesting a broader role for 2B4 in leukocyte activation and possibly a role of immunomodulation in other receptor-ligand interactions to enhance leukocyte activation. 2B4 has been clustered as CD244 in the VIIth HLDA workshop. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of CD244 (2-69) reactivity on peripheral blood lymphocytes analyzed by flow cytometry. Second step staining with Cat. No. 555988.

BD Pharmingen™ Purified Mouse Anti-Human CD244 - Purified - Clone 2-69 - Isotype Mouse IgG2a, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: 2

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-2B4 antibody (ABIN2689111)

Sivori, Parolini, Falco, Marcenaro, Biassoni, Bottino, Moretta, Moretta: "2B4 functions as a co-receptor in human NK cell activation." in: European journal of immunology, Vol. 30, Issue 3, pp. 787-93, (2000) (PubMed).

Nakajima, Cella, Langen, Friedlein, Colonna: "Activating interactions in human NK cell recognition: the role of 2B4-CD48." in: European journal of immunology, Vol. 29, Issue 5, pp. 1676-83, (1999) (PubMed).

Tangye, Lazetic, Woollatt, Sutherland, Lanier, Phillips: "Cutting edge: human 2B4, an activating NK cell receptor, recruits the protein tyrosine phosphatase SHP-2 and the adaptor signaling protein SAP." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 6981-5, (1999) (PubMed).

Mathew, Garni-Wagner, Land, Takashima, Stoneman, Bennett, Kumar: "Cloning and characterization of the 2B4 gene encoding a molecule associated with non-MHC-restricted killing mediated by activated natural killer cells and T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 10, pp. 5328-37, (1993) (PubMed).

Target Details for 2B4

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Alternative Name: CD244 (CD244 Products)

Synonyms: CD244 antibody, 2B4 antibody, NAIL antibody, NKR2B4 antibody, Nmrk antibody, SLAMF4 antibody, C9.1 antibody, F730046O15Rik antibody, Ly90 antibody, CD244 molecule antibody, CD244 natural killer cell receptor 2B4 antibody, CD244 antibody, Cd244 antibody

Background: Synonyms: 2B4