CD27 antibody (Biotin)

Catalog No. ABIN2689133

This Armenian Hamster Monoclonal antibody specifically detects CD27 in FACS. It exhibits reactivity toward Mouse and Rat. It has been mentioned in 6+ publications

Quick Overview for CD27 antibody (Biotin) (ABIN2689133)

Target: CD27 (CD27 Molecule (CD27))

Reactivity: Mouse, Rat

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This CD27 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: LG-3A10

Product Details anti-CD27 Antibody (Biotin)

Characteristics: The LG.3A10 antibody reacts with CD27, a lymphocyte-restricted member of the Tumor Necrosis Factor Receptor family which binds to CD70. The CD27 Molecule is a 45- kDa transmembrane glycoprotein which is constitutively expressed by lymphocytes of the T lineage: virtually all thymocytes and over 90 % of peripheral T cells bearing both αβ and γδ T-cell receptors. CD27 cooperates with the pre-TCR in mediating thymocyte differentiation and expansion. In addition, one to ten percent of mature peripheral B cells express CD27, and CD27's role in the differentiation of human plasma cells has been studied. Mouse NK cells, freshly isolated and IL-2-activated, also express CD27. In the bone marrow, CD27 is found on a progenitor population which provides short-term hematopoietic reconstitution. Cells of the myeloid lineage do not express CD27. Cross-linked LG.3A10 mAb has been reported to amplify the proliferative response of purified T lymphocytes to suboptimal mitogenic stimulation1 and to enhance NK-cell proliferation and IFN-γ production. In contrast, non-cross-linked LG.3A10 mAb inhibits CD3-induced pre-T cell development by interfering with the receptor-ligand interaction. This hamster mAb to a mouse leukocyte antigen has been observed to cross-react with a similar population of rat leukocytes. Expression of CD27 on mouse splenocytes. C57BL/6 splenocytes were stained simultaneously with PE-conjugated anti-mouse CD3e mAb 500A2 (Cat. No. 553240) and biotinylated mAb LG.3A10 (right panel), followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Hamster Anti-Mouse CD27 - Biotin - Clone LG.3A10 - Isotype Armenian Hamster IgG1, κ - Reactivity Ms, Rat - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Armenian hamster fibroblast line ARHO12 transfected with mouse Cd27 cDNA

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD27 antibody (Biotin) (ABIN2689133)

Agematsu, Hokibara, Nagumo, Shinozaki, Yamada, Komiyama: "Plasma cell generation from B-lymphocytes via CD27/CD70 interaction." in: Leukemia & lymphoma, Vol. 35, Issue 3-4, pp. 219-25, (2000) (PubMed).

Takeda, Oshima, Hayakawa, Akiba, Atsuta, Kobata, Kobayashi, Ito, Yagita, Okumura: "CD27-mediated activation of murine NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 4, pp. 1741-5, (2000) (PubMed).

Wiesmann, Phillips, Mojica, Pierce, Searles, Spangrude, Lemischka: "Expression of CD27 on murine hematopoietic stem and progenitor cells." in: Immunity, Vol. 12, Issue 2, pp. 193-9, (2000) (PubMed).

Gravestein, van Ewijk, Ossendorp, Borst: "CD27 cooperates with the pre-T cell receptor in the regulation of murine T cell development." in: The Journal of experimental medicine, Vol. 184, Issue 2, pp. 675-85, (1996) (PubMed).

Gravestein, Nieland, Kruisbeek, Borst: "Novel mAbs reveal potent co-stimulatory activity of murine CD27." in: International immunology, Vol. 7, Issue 4, pp. 551-7, (1995) (PubMed).

Gravestein, Blom, Nolten, de Vries, van der Horst, Ossendorp, Borst, Loenen: "Cloning and expression of murine CD27: comparison with 4-1BB, another lymphocyte-specific member of the nerve growth factor receptor family." in: European journal of immunology, Vol. 23, Issue 4, pp. 943-50, (1993) (PubMed).

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints