Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

CD28 antibody

CD28 Reactivity: Baboon, Cynomolgus, Rhesus Monkey FACS Host: Mouse Monoclonal CD28-2 unconjugated
Catalog No. ABIN2689147
  • Target See all CD28 Antibodies
    CD28
    Reactivity
    • 173
    • 128
    • 58
    • 38
    • 21
    • 20
    • 12
    • 12
    • 7
    • 7
    • 6
    • 5
    • 1
    • 1
    • 1
    • 1
    Baboon, Cynomolgus, Rhesus Monkey
    Host
    • 126
    • 79
    • 33
    • 16
    • 8
    • 3
    • 2
    • 1
    Mouse
    Clonality
    • 188
    • 74
    • 3
    Monoclonal
    Conjugate
    • 88
    • 30
    • 29
    • 21
    • 14
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD28 antibody is un-conjugated
    Application
    • 199
    • 94
    • 47
    • 47
    • 38
    • 31
    • 27
    • 26
    • 19
    • 17
    • 13
    • 7
    • 7
    • 6
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Brand
    BD Pharmingen™
    Characteristics
    The CD28.2 monoclonal antibody specifically binds to CD28, a 44 kDa homodimeric transmembrane glycoprotein present on most mature T cells, thymocytes and plasma cells. CD28 is a costimulatory receptor that binds CD80 and CD86 as ligands and plays a very important role in T cell-B cell interactions. It has been suggested that CD28 initiates and regulates a separate and distinct signal transduction pathway from those stimulated by the TCR complex. Additionally, it has been reported that CD28 antibody clones vary in their ability to stimulate T cells to produce IL-2 and increase intracellular Ca2+ concentration. This finding suggests the existence of functionally distinct subregions on the CD28 Molecule. CD28.2 has been demonstrated to bind to the same molecule as clone L293, another CD28 mAb, and has been reported to induce Ca2+ influx in Jurkat T cells. This clone also cross-reacts with a subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of baboon, rhesus and cynomolgus macaques. The distribution on lymphocytes is similar to that observed with normal human donor lymphocytes, however, as described in the literature, the frequency of CD28.2-positive lymphocytes is reduced in the non-human primate, with the majority of cells being CD28 negative. Profile of anti-CD28 reactivity on peripheral blood lymphocytes of Rhesus macaque (macaca mulatta) analyzed by flow cytometry.

    BD Pharmingen™ Purified Mouse Anti-Human CD28 - Purified - Clone CD28.2 - Isotype Mouse IgG1, κ - Reactivity Bab, Cyno, Rhe - 0.1 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human CD28 Transfected Cell Line
    Clone
    CD28-2
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product CD28 Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C.
  • Target
    CD28
    Alternative Name
    CD28 (CD28 Products)
    Synonyms
    Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody
    Background
    Synonyms: CD28 antigen, T44, Tp44, TP44
    Pathways
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
You are here:
Support