ITGB1 antibody

Catalog No. ABIN2689158

The Mouse Monoclonal anti-ITGB1 antibody has been validated for WB, FACS, ELISA, IP, IF and IHC (fro). It is suitable to detect ITGB1 in samples from Pig. There are 4+ publications available.

Quick Overview for ITGB1 antibody (ABIN2689158)

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: NaM160

Product Details anti-ITGB1 Antibody

Brand: BD Pharmingen™

Characteristics: The NaM160-1A3 antibody reacts with the 116- kDa integrin β1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin α chains. With α1 through α6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with αv (CD51), it forms αvβ1 integrin. As a result, CD29 has a broad tissue distribution, including leukocytes, endothelia, epithelia, and oocytes. Porcine CD29 is believed to be a major target for natural antibodies involved in rejection of pig-to-human xenografts, and a mAb to block recognition of pig CD29 may have therapeutic applications. NaM160-1A3 mAb does not cross-react with human peripheral blood lymphocytes or umbilical cord vein endothelial cells. Expression of CD29 on pig peripheral blood leukocytes. Pig whole blood was stained with either purified mAb NaM160-1A3 (filled histograms) or purified mouse IgG1, κ isotype control mAb MOPC-31C (Cat. No. 557273, open histograms), followed by FITC-conjugated goat anti-mouse Ig (multiple adsorption, Cat. No. 554001). Erythrocytes were lysed (PharM Lyse™, Cat. No. 555899), non-viable leukocytes were excluded by staining with 7-AAD (Via-Probe™, Cat. No. 555816/555815), and lymphocytes (left panel), monocytes (center panel), and granulocytes (right panel) were gated according to their light-scatter profiles. Multi-color staining (data not shown) demonstrates that most CD4-CD8+ T and NK cells and most CD4+CD8+ memory T cells make up the CD29bright lymphocyte population, CD4+CD8- T lymphocytes are CD29dim, and the CD4-CD8- lymphocytes have CD29negative and CD29dim subpopulations. Flow cytometry was performed on a FACSCalibur™ .

BD Pharmingen™ Purified Mouse Anti-Pig CD29 - Purified - Clone NaM160-1A3 - Isotype Mouse IgG1, κ - Reactivity Pig - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Pig platelets

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-ITGB1 antibody (ABIN2689158)

Jiménez-Marín, Garrido, de Andrés-Cara, Morera, Barbancho, Llanes: "Molecular cloning and characterization of the pig homologue to human CD29, the integrin beta1 subunit." in: Transplantation, Vol. 70, Issue 4, pp. 649-55, (2000) (PubMed).

Linfor, Berger: "Potential role of alphav and beta1 integrins as oocyte adhesion molecules during fertilization in pigs." in: Journal of reproduction and fertility, Vol. 120, Issue 1, pp. 65-72, (2000) (PubMed).

Richard, Thibaudeau, Charreau, Loirat, Naulet, Blanchard, Soulillou, Bouhours: "Characterization of a murine monoclonal antibody specific for swine beta1 integrin." in: Xenotransplantation, Vol. 5, Issue 1, pp. 75-83, (1998) (PubMed).

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, (1990) (PubMed).

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: CD29

Background: Synonyms: Integrin β1 chain

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome