CD3 antibody

Catalog No. ABIN2689163

This Mouse Monoclonal antibody specifically detects CD3 in FACS, IP, IHC (fro), CyTox and FM. It exhibits reactivity toward Rat. It has been mentioned in 4+ publications

Quick Overview for CD3 antibody (ABIN2689163)

Target: CD3

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox), Fluorescence Microscopy (FM)

Clone: 1F4

Product Details anti-CD3 Antibody

Characteristics: The 1F4 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. In vitro, immobilized 1F4 mAb induces T-cell proliferation, and soluble mAb synergizes with PMA for T-cell activation. Pre-incubation of splenocytes with mAb 1F4 blocks staining with mAb G4.18 (Cat. No. 559974/554830). This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. CD3e expression in spleen and thymus. LEW splenocytes were preincubated with Rat BD Fc Block™ purified anti-rat CD32 mAb D34-485 (Cat. No. 550270/550271) and simultaneously stained with FITC-onjugated anti-rat CD5 mAb OX-19 (Cat. No. 551449, left panels) and purified mAb 1F4 (middle left panel), followed by PE-conjugated anti-mouse IgMa mAb DS-1 (Cat. No. 553517, left panels). LEW thymocytes were stained with purified 1F4 mAb (far right panel) followed by PE-conjugated anti-mouse IgMa mAb DS-1 (right panels). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Purified Mouse Anti-Rat CD3 - Purified - Clone 1F4 - Isotype Mouse IgM, κ - Reactivity Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: F344 rat splenic T cells stimulated with PMA plus A23187

Isotype: IgM kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD3 antibody (ABIN2689163)

Naper, Vaage, Lambracht, Løvik, Butcher, Wonigeit, Rolstad: "Alloreactive natural killer cells in the rat: complex genetics of major histocompatibility complex control." in: European journal of immunology, Vol. 25, Issue 5, pp. 1249-56, (1995) (PubMed).

Elbe, Kilgus, Hünig, Stingl: "T-cell receptor diversity in dendritic epidermal T cells in the rat." in: The Journal of investigative dermatology, Vol. 102, Issue 1, pp. 74-9, (1994) (PubMed).

Nicolls, Aversa, Pearce, Spinelli, Berger, Gurley, Hall: "Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody." in: Transplantation, Vol. 55, Issue 3, pp. 459-68, (1993) (PubMed).

Tanaka, Masuko, Yagita, Tamura, Hashimoto: "Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2791-5, (1989) (PubMed).

Target Details for CD3

Target: CD3

Alternative Name: CD3

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway